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AB279769

Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit

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Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (AB279769)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (AB279769)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated with Calyculin A and Pervanadate.

Sandwich ELISA - Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (AB279769)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (AB279769)

Positive Control.

Jurkat cells were treated with Calyculin A and Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (AB279769)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-CD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (AB279769)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated with Calyculin A and Pervanadate.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

PhosphoCD167a/DDR1 (Y792) and Total CD167a/DDR1 ELISA Kit (ab279769) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated CD167a/DDR1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-CD167a/DDR1 (Y792) and total CD167a/DDR1. An anti-pan CD167a/DDR1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and CD167a/DDR1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho CD167a/DDR1 (Y792) antibody is added to detect phosphorylated CD167a/DDR1. In the remaining wells, rabbit anti-pan-CD167a/DDR1 antibody is used to detect pan CD167a/DDR1 . After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD167a/DDR1 (Y792) or pan CD167a/DDR1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD167a also known as DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase with a molecular mass of approximately 101 kDa. This protein is expressed widely in various tissues including the thyroid lung kidney and mammary gland. It becomes activated upon collagen binding leading to the regulation of cell adhesion proliferation and extracellular matrix remodeling. The DDR1 protein plays a mechanistic role in responding to unique collagen-rich environments within the body.
Biological function summary

CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.

Pathways

CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.

Mutations or irregular expression of CD167a/DDR1 have implications in cancer and fibrotic diseases. Overexpression or aberration of DDR1 can contribute to tumorigenesis facilitating tumor cell invasion and metastasis. In fibrotic conditions altered DDR1 activity can disrupt normal matrix remodeling linking it to proteins such as collagen I and III which play major roles in these disorders. Understanding DDR1's role offers potential for therapeutic interventions in targeting related pathological conditions.
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Product protocols

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Target data

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websiteProtocolBooklet
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