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AB279763

Phospho-CREB (S133) ELISA Kit

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Phospho-CREB (S133) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-CREB (S133) in Human in Cell/Tissue Extracts samples.

View Alternative Names

Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1

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Sandwich ELISA - Phospho-CREB (S133) ELISA Kit (AB279763)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-CREB (S133) ELISA Kit (AB279763)

Positive Control.

HeLa cells were treated with PMA at 37°C for 20 min.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-CREB (S133) ELISA Kit (AB279763)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-CREB (S133) ELISA Kit (AB279763)

HeLa cells treated/untreated with PMA. HeLa cells were untreated or treated with 250 nM PMA for 20 mins.

Sandwich ELISA - Phospho-CREB (S133) ELISA Kit (AB279763)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-CREB (S133) ELISA Kit (AB279763)

HeLa cells treated/untreated with PMA. HeLa cells were untreated or treated with 250 nM PMA for 20 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-CREB (S133) ELISA Kit (ab279763) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated CREB protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-CREB. An anti-pan CREB antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and CREB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-CREB (S133) antibody is used to detect phosphorylated CREB (S133). After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CREB (S133) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279763-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279763-CMP06", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279763-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279763-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279763-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279763-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279763-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-CREB (S133)-antibody", "number":"AB279763-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-CREB Coated Microplate", "number":"AB279763-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279763-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.
Biological function summary

CREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.

Pathways

CREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.

Research links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.
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Product protocols

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Target data

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed : 14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
See full target information CREB phospho S133
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websiteProtocolBooklet
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