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AB279770

Phospho-DDR2 (Y740) ELISA Kit

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Phospho-DDR2 (Y740) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-DDR2 (Y740) in Human in Cell/Tissue Extracts samples.

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CD167b, NTRKR3, TKT, TYRO10, DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10

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Sandwich ELISA - Phospho-DDR2 (Y740) ELISA Kit (AB279770)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-DDR2 (Y740) ELISA Kit (AB279770)

Positive Control.

Jurkat cells were treated with PV (Pervanadate).

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-DDR2 (Y740) ELISA Kit (AB279770)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-DDR2 (Y740) ELISA Kit (AB279770)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated or untreated with Calyculin A and Pervanadate.

Sandwich ELISA - Phospho-DDR2 (Y740) ELISA Kit (AB279770)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-DDR2 (Y740) ELISA Kit (AB279770)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated or untreated with Calyculin A and Pervanadate.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-DDR2 (Y740) ELISA Kit (ab279770) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated DDR2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-DDR2 (Y740). An anti-pan DDR2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and DDR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-DDR2 (Y740) antibody is used to detect phosphorylated DDR2 (Y740). After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DDR2 (Y740) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279770-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279770-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279770-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279770-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279770-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-DDR2 (Y740)-antibody", "number":"AB279770-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-DDR2 Coated Microplate", "number":"AB279770-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279770-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated Jurkat cell lysate", "number":"AB279770-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279770-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Discoidin Domain Receptor 2 (DDR2) also known as Tyrosine-protein kinase DDR2 is a receptor tyrosine kinase that binds to collagen initiating various cellular processes. It has a molecular mass of approximately 97 kDa. This receptor is expressed in various tissues including skin bone and cartilage as well as in certain cancer cells. DDR2 activation is achieved through the recognition of specific motifs such as GFOGER in collagen leading to signaling cascades that impact cell functions like adhesion proliferation and extracellular matrix remodeling.
Biological function summary

DDR2 functions as an integral part of the cellular response to collagen. The receptor plays a critical role in the development and maintenance of connective tissues. It exists as a monomer but can form complexes upon ligand binding facilitating its activity. DDR2 signaling influences critical processes such as wound healing and tissue homeostasis by modulating matrix metalloproteinases (MMPs) activity which are important for remodeling the extracellular matrix.

Pathways

DDR2 abettors collagen-induced signal transduction particularly within the mitogen-activated protein kinase (MAPK) pathway and the Src family kinase pathway. These pathways involve proteins like SHP2 and Src both of which interact with DDR2 to propagate cellular signals. DDR2 activation leads to phosphorylation events that stimulate cytoskeletal rearrangements and gene expression modulations relevant to cellular growth and differentiation.

DDR2 is linked to fibrosis and cancer. The receptor's overexpression or aberrant signaling can contribute to fibrotic diseases characterized by excessive scarring by promoting collagen deposition and fibroblast proliferation. In cancer DDR2 dysregulation often associates with tumor progression and metastasis particularly in lung cancer and sarcomas. This links DDR2 with other oncogenic proteins and pathways including those governed by MMP proteins which are involved in tumor invasion and metastasis.
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Product protocols

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Target data

Tyrosine kinase involved in the regulation of tissues remodeling (PubMed : 30449416). It functions as a cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
See full target information DDR2 phospho Y740
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