Phospho-EIF2S1 (S52) and Total EIF2S1 ELISA Kit

Product details

Phospho-EIF2S1 (S52) and Total EIF2S1 ELISA Kit (ab279775) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated EIF2S1 (S52) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-EIF2S1 and total EIF2S1. An anti-pan EIF2S1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and EIF2S1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, biotinylated anti-phospho-eIF-2A (S52) antibody is added to detect phosphorylated EIF2S1. In the remaining wells, rabbit anti-pan-eIF-2A antibody is used to detect pan EIF2S1. After washing away unbound antibody, HRP-Streptavidin or HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EIF2S1 (S52) or pan EIF2S1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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