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AB279774

Phospho-EIF2S1 (S52) ELISA Kit

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Phospho-EIF2S1 (S52) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-EIF2S1 (S52) in Human in Cell/Tissue Extracts samples.

View Alternative Names

EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha

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Sandwich ELISA - Phospho-EIF2S1 (S52) ELISA Kit (AB279774)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-EIF2S1 (S52) ELISA Kit (AB279774)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated or untreated with Calyculin A and Pervanadate.

Sandwich ELISA - Phospho-EIF2S1 (S52) ELISA Kit (AB279774)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-EIF2S1 (S52) ELISA Kit (AB279774)

Positive Control.

HeLa cells were treated with Calyculin A and TNF-A.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-EIF2S1 (S52) ELISA Kit (AB279774)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-EIF2S1 (S52) ELISA Kit (AB279774)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated or untreated with Calyculin A and Pervanadate.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-EIF2S1 (S52) ELISA Kit (ab279774) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated EIF2S1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-EIF2S1. An anti-pan EIF2S1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and eIF2alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phospho-eIF-2A (S52) antibody is used to detect phosphorylated EIF2S1. After washing away unbound antibody, HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of eIF2alpha (S52) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279774-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279774-CMP09", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279774-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279774-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279774-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-EIF2S1 Coated Microplate", "number":"AB279774-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-phospho-EIF2S1 (S52)-antibody", "number":"AB279774-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279774-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279774-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - HeLa cell lysate", "number":"AB279774-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary

EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.

Pathways

EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.

EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.
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Product protocols

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Target data

Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed : 16289705, PubMed : 38340717). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC) (PubMed : 16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed : 16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed : 16289705). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress : phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed : 19131336, PubMed : 33384352, PubMed : 38340717). EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage : phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy (PubMed : 38340717).
See full target information EIF2S1 phospho S52
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websiteProtocolBooklet
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