Phospho-eNOS (S1177) and Total eNOS ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-eNOS (S1177) and Total eNOS in Human in Cell Lysate samples.
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- Frontiers in cardiovascular medicine 10:12222432023Plasmacytoid dendritic cells contribute to vascular endothelial dysfunction in type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Alluri,B Srinivas,S Belmadani,K MatrouguiPubMed 38094119
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate
- Assay type
Semi-quantitative
- Reactive species
Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-eNOS (S1177) and Total eNOS ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-eNOS (S1177) and Total eNOS in Human in Cell Lysate samples.
Key facts
- Detection method
Colorimetric
- Sample types
Cell Lysate
- Assay type
Semi-quantitative
- Reactive species
Human
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Phospho-eNOS (S1177) and Total eNOS (ab279779) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated eNOS protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-eNOS and total eNOS. An anti-pan eNOS antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and eNOS present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho eNOS (S1177) antibody is added to detect phosphorylated eNOS. In the remaining wells, mouse anti-pan-eNOS antibody is used to detect pan eNOS. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of eNOS (S1177) or pan eNOS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.
- Biological function summary
ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.
- Pathways
ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.
- Associated diseases and disorders
ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.
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3 product images
Sandwich ELISA - Phospho-eNOS (S1177) and Total eNOS ELISA Kit (ab279779)
Positive Control.
HUVEC cells were treated with PV (pervanadate).
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-eNOS (S1177) and Total eNOS ELISA Kit (ab279779)
THP-1 cells untreated/treated with Pervanadate. THP-1 cells were untreated or treated with PV (pervanadate).
Sandwich ELISA - Phospho-eNOS (S1177) and Total eNOS ELISA Kit (ab279779)
THP-1 cells untreated/treated with Pervanadate. THP-1 cells were untreated or treated with PV (pervanadate).
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