JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279791

Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 in Human in Cell/Tissue Extracts samples.
3 Images
Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit (AB279791)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit (AB279791)

Positive Control.

A431 cells were treated with recombinant human EGF at 37°C for 20 min.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit (AB279791)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit (AB279791)

A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml recombinant human EGF for 20 mins.

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit (AB279791)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 ELISA Kit (AB279791)

A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml recombinant human EGF for 20 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phospho-ErbB3 / HER3 (Y1262) and Total ErbB3 / HER3 (ab279791) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB3 / HER3 (Y1262) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and phospho-ErbB3 / HER3 (Y1262) and total ErbB3 / HER3. An anti-pan ErbB3 / HER3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ErbB3 / HER3 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho ErbB3 / HER3 (Y1262) antibody is added to detect phosphorylated ErbB3 / HER3 (Y1262). In the remaining wells, biotinylated anti-pan-ErbB3 / HER3 antibody is used to detect pan ErbB3 / HER3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB3 / HER3 (Y1262) or pan ErbB3 / HER3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279791-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279791-CMP05", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279791-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279791-CMP10", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279791-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279791-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279791-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ErbB3 / HER3 Coated Microplate", "number":"AB279791-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-ErbB3 / HER3 detection antibody", "number":"AB279791-CMP11", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-ErbB3 / HER3 (Y1262)-antibody", "number":"AB279791-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - A431 cell lysate", "number":"AB279791-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279791-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.
Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB279787

Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit

Sandwich ELISA - Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit (AB279787)

  • 0
  • 0
Assay Kits

AB279809

Phospho-Fyn (Tyr530) and Total Fyn ELISA Kit

Sandwich ELISA - Phospho-Fyn (Tyr530) and Total Fyn ELISA Kit (AB279809)

  • 0
  • 0
Assay Kits

AB279844

Phospho-c-Jun (S63) and Total c-Jun ELISA Kit

Sandwich ELISA - Phospho-c-Jun (S63) and Total c-Jun ELISA Kit (AB279844)

  • 0
  • 0
Assay Kits

AB279751

Phospho-BTK (Y551) ELISA Kit

Sandwich ELISA - Phospho-BTK (Y551) ELISA Kit (AB279751)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com