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AB279790

Phospho-ErbB3 / HER3 (Y1262) ELISA Kit

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Phospho-ErbB3 / HER3 (Y1262) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-ErbB3 / HER3 (Y1262) in Human in Cell/Tissue Extracts samples.

View Alternative Names

HER3, ERBB3, Receptor tyrosine-protein kinase erbB-3, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3

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Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (AB279790)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (AB279790)

Positive Control.

A431 cells were treated with recombinant human EGF at 37°C for 20 min.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (AB279790)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (AB279790)

A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml recombinant human EGF for 20 mins.

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (AB279790)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (AB279790)

A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml recombinant human EGF for 20 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-ErbB3 / HER3 (Y1262) ELISA Kit (ab279790) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB3 / HER3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ErbB3 / HER3. An anti-pan ErbB3 / HER3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ErbB3 / HER3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-ErbB3 / HER3 (Y1262) antibody is used to detect phosphorylated ErbB3 / HER3. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB3 / HER3 (Y1262) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279790-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Positive Control - A431 cell lysate", "number":"AB279790-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279790-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279790-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ErbB3 / HER3 Coated Microplate", "number":"AB279790-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-ErbB3 / HER3 (Y1262)-antibody", "number":"AB279790-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279790-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279790-CMP09", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279790-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279790-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB3 protein also known as HER3 is a member of the epidermal growth factor receptor (EGFR) family. It belongs to the ErbB receptor tyrosine kinase family but lacks intrinsic kinase activity. The HER3 protein has a molecular weight of approximately 180 kDa. You will find HER3 expressed in various tissues with high expression in the epithelial tissues where it plays a role in normal cellular processes. HER3 interacts with multiple ligands facilitating cellular communication and growth regulation.
Biological function summary

HER3 is essential for activating important signaling pathways that regulate proliferation differentiation and survival. The ErbB3 protein forms heterodimers with other ErbB family members such as ErbB2 (HER2) which is necessary for its signaling capability. The HER3 protein is abundant in heart and nervous tissues indicating its role in cardiovascular and neurological functions. Its activation modulates gene expression through downstream effectors mediated by dimerization.

Pathways

HER3 plays a pivotal role in the PI3K/Akt and MAPK signaling pathways. These pathways are fundamental in cellular responses like growth and survival. HER3 serves as a major partner for HER2 in these signaling cascades enhancing downstream signaling effects. HER3's connection to these pathways makes it a critical node collaborating closely with proteins such as HER2 and EGFR to maintain efficient communication and function within these networks.

HER3 associates strongly with cancer and cardiovascular diseases. Its overexpression in certain cancers such as breast cancer leads to enhanced tumor growth and resistance to therapy. HER3’s relationship with the HER2 protein is especially significant in HER2-positive breast cancer where their interaction drives tumor progression. Additionally alterations in HER3 expression or function impact cardiovascular diseases as it plays a significant role in heart and vascular cell behavior. Recognizing HER3's involvement in these conditions allows for targeted therapeutic approaches and improves disease management strategies.
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Product protocols

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Target data

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed : 20682778). May also be activated by CSPG5 (PubMed : 15358134). Involved in the regulation of myeloid cell differentiation (PubMed : 27416908).
See full target information ERBB3 phospho Y1262
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websiteProtocolBooklet
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