Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha (ab279787) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human and cell lysates. By determining phosphorylated Estrogen receptor alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Estrogen receptor alpha (S118) and total Estrogen receptor alpha. An anti-pan Estrogen receptor alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Estrogen receptor alpha present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho-ER-alpha (S118) antibody is added to detect phosphorylated Estrogen receptor alpha. In the remaining wells, mouse anti-pan-ER alpha antibody is used to detect pan Estrogen receptor alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Estrogen receptor alpha (S118) or pan Estrogen receptor alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.
- Biological function summary
ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.
- Pathways
ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.
- Associated diseases and disorders
ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.
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3 product images
Sandwich ELISA - Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit (ab279787)
Positive Control.
ZR751 cells were treated with b-estradiol at 37°C for 1 hours.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Sandwich ELISA - Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit (ab279787)
ZR751 cells untreated/treated with beta-estradiol. ZR751 cells were untreated or treated with beta-estradiol for 1 hour.
Sandwich ELISA - Phospho-Estrogen receptor alpha (S118) and Total Estrogen receptor alpha ELISA Kit (ab279787)
ZR751 cells untreated/treated with beta-estradiol. ZR751 cells were untreated or treated with beta-estradiol for 1 hour.
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