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AB279799

Phospho-Flt3 / CD135 (Y589) ELISA Kit

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Phospho-Flt3 / CD135 (Y589) ELISA Kit is a ELISA for the measurement of Phospho-Flt3 / CD135 (Y589) in Human in Cell/Tissue Extracts samples.

View Alternative Names

CD135, FLK2, STK1, FLT3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, FLK-2, FLT-3, STK-1

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Sandwich ELISA - Phospho-Flt3 / CD135 (Y589) ELISA Kit (AB279799)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Flt3 / CD135 (Y589) ELISA Kit (AB279799)

A431 cells treated with/without pervanadate. A431 cells were treated or untreated with Pervanadate.

Sandwich ELISA - Phospho-Flt3 / CD135 (Y589) ELISA Kit (AB279799)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Flt3 / CD135 (Y589) ELISA Kit (AB279799)

A431 cells treated with/without pervanadate. A431 cells were treated or untreated with Pervanadate.

Sandwich ELISA - Phospho-Flt3 / CD135 (Y589) ELISA Kit (AB279799)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Flt3 / CD135 (Y589) ELISA Kit (AB279799)

Positive Control.

Jurkat cells were treated with Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Phospho-Flt3 / CD135 (Y589) ELISA Kit (ab279799) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated FLT3 / CD135 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-FLT3 / CD135. An anti-pan Flt3 / CD135 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Flt3 / CD135 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-FLT3 / CD135 (Y589) antibody is used to detect phosphorylated Flt3 / CD135. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Flt3 / CD135 (Y589) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Flt3 also known as CD135 or FLT3 tyrosine kinase is a receptor tyrosine kinase that belongs to the class III receptor tyrosine kinase family. It has a molecular weight of approximately 160 kDa. This protein plays an important role in the development of hematopoietic stem and progenitor cells. Flt3 is expressed mainly in early progenitor cells within the bone marrow. Additionally it is present in some populations of mature cells in the peripheral blood and lymphoid organs.
Biological function summary

Flt3 functions as a receptor that is activated by binding to its ligand FLT3 ligand (FLT3L). This interaction stimulates the associated tyrosine kinase activity leading to auto-phosphorylation of Flt3 and subsequent downstream signaling. Flt3 does not form a heterocomplex rather it dimerizes upon ligand binding to initiate signaling cascades. These cascades promote cell proliferation differentiation and survival particularly impacting hematopoiesis and immune responses.

Pathways

Flt3 signaling impacts the internal signaling networks involving the MAPK/ERK and PI3K/AKT pathways both central to cell cycle regulation and apoptosis. Flt3 interacts functionally with proteins such as SHP2 and GRB2 in these pathways aiding signal transduction. The Flt3 receptor also shares functional motifs with KIT and PDGF receptors indicating shared regulatory mechanisms involved in cellular proliferation.

Flt3 mutations especially internal tandem duplications in Flt3/ITD are significant in acute myeloid leukemia (AML) leading to abnormal cell growth and survival. These mutations often co-occur with other genetic anomalies such as NPM1 mutations contributing to the oncogenic process. The abnormal activation of Flt3 kinase activity also has ramifications in myelodysplastic syndromes making it a target for therapeutic intervention with drugs such as anti-FLT3 inhibitors in the treatment regime.
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Product protocols

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
See full target information FLT3 phospho Y589
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websiteProtocolBooklet
en
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Product promise

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