Phospho-FOXO3A (Ser253) and Total FOXO3A ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-FOXO3A (Ser253) and Total FOXO3A in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-FOXO3A (Ser253) and Total FOXO3A ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-FOXO3A (Ser253) and Total FOXO3A in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-FOXO3A (S253) and Total FOXO3A (ab279802) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated FOXO3A protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-FOXO3A and total FOXO3A. An anti-pan FOXO3A antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and FOXO3A present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho FOXO3A (S253) antibody is added to detect phosphorylated FOXO3A. In the remaining wells, biotinylated anti-pan-FOXO3A antibody is used to detect pan FOXO3A. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FOXO3A (S253) or pan FOXO3A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FOXO3A also known as FOXO3 or 1e2 is a transcription factor with a molecular weight of approximately 71 kDa. It belongs to the Forkhead box O family of proteins. This protein plays an important role in the regulation of transcription in response to oxidative stress and growth stimuli. FOXO3A is expressed in a variety of tissues including liver muscle and brain where it contributes to a wide range of cellular processes related to stress resistance and metabolism. Scientists frequently study FOXO3A using techniques like FOXO3A ELISA and apply FOXO3A anticuerpos in research to understand its function more intricately.
- Biological function summary
FOXO3A acts as a regulator of genes involved in cell cycle arrest apoptosis and DNA repair. It is not part of a simple protein complex but works closely with multiple factors to influence cellular homeostasis. Significantly FOXO3A controls the expression of Bcl-2 a protein important for cell survival and the protein p27 which is important for cell cycle control. FOXO3A's activity modulates cellular longevity and impacts stress responses highlighting its abundant biological functions.
- Pathways
FOXO3A integrates into the PI3K/AKT and the insulin signaling pathways both essential for cellular metabolism and proliferation control. In the PI3K/AKT pathway FOXO3A functionally links with proteins such as AKT and PTEN where its activity can be inhibited by AKT-mediated phosphorylation. In doing so it supports a balance between cell survival and apoptosis allowing the cell to adjust to growth conditions and external stressors effectively.
- Associated diseases and disorders
FOXO3A is linked to cancer and neurodegenerative diseases. In cancer alterations in FOXO3A activity or expression can affect tumor growth and resistance to oxidative stress often involving interactions with proteins like MDM2. Similarly in neurodegenerative disorders such as Alzheimer's disease FOXO3A modifies responses to oxidative damage potentially tied to proteins like Tau. Both instances highlight the role of FOXO3A as a versatile modulator that can influence the progression and management of various diseases.
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3 product images
Sandwich ELISA - Phospho-FOXO3A (Ser253) and Total FOXO3A ELISA Kit (ab279802)
Positive Control.
HeLa cells were treated with Calyculin A.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-FOXO3A (Ser253) and Total FOXO3A ELISA Kit (ab279802)
HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.
Sandwich ELISA - Phospho-FOXO3A (Ser253) and Total FOXO3A ELISA Kit (ab279802)
HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.
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