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AB279805

Phospho-FRS2 (Y436) ELISA Kit

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Phospho-FRS2 (Y436) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-FRS2 (Y436) in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Frs2a, Fibroblast growth factor receptor substrate 2, FGFR substrate 2, FGFR-signaling adaptor SNT, FRS2-alpha, Suc1-associated neurotrophic factor target 1, SNT-1

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Sandwich ELISA - Phospho-FRS2 (Y436) ELISA Kit (AB279805)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-FRS2 (Y436) ELISA Kit (AB279805)

NIH/3T3 cells treated with/without pervanadate. NIH/3T3 cells were treated with Pervanadate.

Sandwich ELISA - Phospho-FRS2 (Y436) ELISA Kit (AB279805)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-FRS2 (Y436) ELISA Kit (AB279805)

Positive Control.

NIH/3T3 cells were treated with Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-FRS2 (Y436) ELISA Kit (AB279805)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-FRS2 (Y436) ELISA Kit (AB279805)

NIH/3T3 cells treated with/without pervanadate. NIH/3T3 cells were treated with Pervanadate.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-FRS2 (Y436) ELISA Kit (ab279805) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated FRS2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-FRS2. An anti-pan FRS2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and FRS2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-FRS2 (Y436) antibody is used to detect phosphorylated FRS2. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FRS2 (Y436) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279805-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279805-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279805-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279805-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279805-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-FRS2 (Y436)-antibody", "number":"AB279805-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-FRS2 Coated Microplate", "number":"AB279805-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated NIH/3T3 cell lysate", "number":"AB279805-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279805-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279805-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRS2 also known as Fibroblast growth factor receptor substrate 2 functions as an adaptor protein in cellular signal transduction. This protein with a molecular mass of approximately 60 kDa plays a critical role in linking receptor tyrosine kinases specifically FGFR (Fibroblast Growth Factor Receptor) to downstream signaling pathways. FRS2 primarily expresses in various tissues with notable expression in neural and epithelial cells. Its function involves phosphorylation and the recruitment of downstream signaling molecules which propagate cellular response signals.
Biological function summary

FRS2 integrates into several signaling complexes that regulate key cellular processes. It acts as a scaffold protein creating platforms for the assembly of signaling complexes such as those involving Grb2 (Growth factor receptor-bound protein 2) and Sos (Son of Sevenless). These complexes mediate processes related to cell differentiation proliferation and survival. The protein's ability to anchor and direct these signaling molecules highlights its importance within cellular mechanisms where it influences signaling cascades leading to specific cellular outcomes.

Pathways

The role of FRS2 is essential in the FGFR and MAPK/ERK pathways. Within these pathways FRS2 interacts closely with proteins like FGFR facilitating their activation and consequent downstream signaling events. Through these interactions the protein influences pathways involved in developmental processes and cellular maintenance. FRS2 by enabling FGFR signal transduction significantly impacts cellular responses to external growth stimuli making it an important player in normal cellular function and development.

Abnormalities in FRS2 function or expression link to certain pathologies including cancer and neurological disorders. For instance overactivation of FRS2-mediated signaling pathways can contribute to oncogenic processes leading to uncontrolled cell growth and proliferation. Furthermore mutations affecting FRS2 interaction with FGFR have implications in developmental disorders characterized by neural defects. Understanding FRS2 connections in these diseases helps in identifying potential therapeutic targets for treatment interventions.
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Product protocols

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Target data

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.
See full target information Frs2 phospho Y436
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websiteProtocolBooklet
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