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AB279817

Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit

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Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit is a ELISA for the measurement of Phospho-gamma H2A.X (S139) and Total gamma H2A.X in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (AB279817)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (AB279817)

Positive Control.

Jurkat cells were treated with CPT at 37°C for 16 hours.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (AB279817)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (AB279817)

Jurkat cells treated with/without CPT. Jurkat cells were untreated or treated with CPT for 16 hours.

Sandwich ELISA - Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (AB279817)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (AB279817)

Jurkat cells treated with/without CPT. Jurkat cells were untreated or treated with CPT for 16 hours.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-gamma H2A.X (S139) and Total gamma H2A.X ELISA Kit (ab279817) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated gamma H2A.X protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-gamma H2A.X and total gamma H2A.X. An anti-pan gamma H2A.X antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and gamma H2A.X present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho gamma H2A.X (S139) antibody is added to detect phosphorylated gamma H2A.X (S139). In the remaining wells, rabbit anti-pan-gamma H2A.X antibody is used to detect pan gamma. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of gamma H2A.X (S139) or pan gamma H2A.X bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279817-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Pan-gamma H2A.X Coated Microplate", "number":"AB279817-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-pan-gamma H2A.X detection antibody", "number":"AB279817-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279817-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279817-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-gamma H2A.X (S139)-antibody", "number":"AB279817-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279817-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279817-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279817-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279817-CMP07", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279817-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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