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AB279810

Phospho-Glucocorticoid Receptor (S226) ELISA Kit

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Phospho-Glucocorticoid Receptor (S226) ELISA Kit is a ELISA for the measurement of Phospho-Glucocorticoid Receptor (S226) in Human in Cell/Tissue Extracts samples.

View Alternative Names

GRL, NR3C1, Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1

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Sandwich ELISA - Phospho-Glucocorticoid Receptor (S226) ELISA Kit (AB279810)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Glucocorticoid Receptor (S226) ELISA Kit (AB279810)

A549 cells treated with Calyculin A and PMA. A549 cells were treated with Calyculin A and PMA.

Sandwich ELISA - Phospho-Glucocorticoid Receptor (S226) ELISA Kit (AB279810)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Glucocorticoid Receptor (S226) ELISA Kit (AB279810)

A549 cells treated with Calyculin A and PMA. A549 cells were treated with Calyculin A and PMA.

Sandwich ELISA - Phospho-Glucocorticoid Receptor (S226) ELISA Kit (AB279810)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Glucocorticoid Receptor (S226) ELISA Kit (AB279810)

Positive Control.

A431 cells were treated with recombinant human EGF at 37°C for 20 min.

Cells were solubilzed at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-Glucocorticoid Receptor (S226) ELISA Kit (ab279810) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Glucocorticoid Receptor protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Glucocorticoid Receptor. An anti-pan Glucocorticoid Receptor antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Glucocorticoid Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and rabbit anti-phospho-Glucocorticoid Receptor (S226) antibody is used to detect phosphorylated Glucocorticoid Receptor. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Glucocorticoid Receptor (S226) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279810-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279810-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279810-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279810-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-Glucocorticoid Receptor (S226)-antibody", "number":"AB279810-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-Glucocorticoid Receptor Coated Microplate", "number":"AB279810-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279810-CMP08", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279810-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - A431 cell lysate", "number":"AB279810-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279810-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The glucocorticoid receptor (GR) also known as the cortisol receptor is a type of nuclear receptor that functions as a transcription factor. This receptor has a molecular mass of approximately 97 kDa. GR is expressed in various tissues including the liver lung and immune cells. Its mechanical action involves binding to glucocorticoids—hormones like cortisol—which can regulate DNA transcription. When activated the receptor translocates into the cell nucleus where it can directly interact with DNA to regulate gene expression.
Biological function summary

The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.

Pathways

The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.

Abnormal glucocorticoid receptor function links to conditions like Cushing's syndrome and glucocorticoid resistance. Cushing's syndrome characterized by excessive cortisol levels shows altered GR signaling. Similarly glucocorticoid resistance involves mutations or dysfunctions in GR that lead to improper hormone action affecting inflammation and immune responses. These diseases often involve other proteins such as various cytokines in the inflammatory response showing the broad impact of GR in disease processes.
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Product protocols

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Target data

Receptor for glucocorticoids (GC) (PubMed : 27120390, PubMed : 37478846). Has a dual mode of action : as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors (PubMed : 28139699). Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed : 9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed : 25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).. Isoform Alpha. Has transcriptional activation and repression activity (PubMed : 11435610, PubMed : 15769988, PubMed : 15866175, PubMed : 17635946, PubMed : 19141540, PubMed : 19248771, PubMed : 20484466, PubMed : 21664385, PubMed : 23820903). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127). Promotes accurate chromosome segregation during mitosis (PubMed : 25847991). May act as a tumor suppressor (PubMed : 25847991). May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity).. Isoform Beta. Acts as a dominant negative inhibitor of isoform Alpha (PubMed : 20484466, PubMed : 7769088, PubMed : 8621628). Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed (PubMed : 19248771, PubMed : 26711253). Loses this transcription modulator function on its own (PubMed : 20484466). Has no hormone-binding activity (PubMed : 8621628). May play a role in controlling glucose metabolism by maintaining insulin sensitivity (By similarity). Reduces hepatic gluconeogenesis through down-regulation of PEPCK in an isoform Alpha-dependent manner (PubMed : 26711253). Directly regulates STAT1 expression in isoform Alpha-independent manner (PubMed : 26711253).. Isoform Alpha-2. Has lower transcriptional activation activity than isoform Alpha. Exerts a dominant negative effect on isoform Alpha trans-repression mechanism (PubMed : 20484466).. Isoform GR-P. Increases activity of isoform Alpha.. Isoform Alpha-B. More effective than isoform Alpha in transcriptional activation, but not repression activity.. Isoform 10. Has transcriptional activation activity.. Isoform Alpha-C1. Has transcriptional activation activity.. Isoform Alpha-C2. Has transcriptional activation activity.. Isoform Alpha-C3. Has highest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127). Mediates glucocorticoid-induced apoptosis (PubMed : 23303127, PubMed : 23820903).. Isoform Alpha-D1. Has transcriptional activation activity.. Isoform Alpha-D2. Has transcriptional activation activity.. Isoform Alpha-D3. Has lowest transcriptional activation activity of all isoforms created by alternative initiation (PubMed : 15866175, PubMed : 23820903). Has transcriptional repression activity (PubMed : 23303127).
See full target information NR3C1 phospho S226
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