AB279815

Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit

Name: Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (ab279815) | Abcam
Brand: Abcam Limited
SKU: AB279815
Price: 1140 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-GSK3 beta (S9) and Total GSK3 beta in Mouse in Cell/Tissue Extracts samples.

3 Images

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

sELISA

Supplier Data

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

Positive Control.

NIH/3T3 cells were treated with recombinant human PDGFBB at 37°C for 10 mins.

Cells were solubilzed at 4 x 10⁷ cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

NIH/3T3 cells treated with/without PDGF. NIH 3T3 cells were untreated or treated with 50 ng/ml recombinant human PDGF BB for 10 mins.

sELISA

Supplier Data

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

NIH/3T3 cells treated with/without PDGF. NIH 3T3 cells were untreated or treated with 50 ng/ml recombinant human PDGF BB for 10 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (ab279815) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated GSK3 beta protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-GSK3 beta and total GSK3 beta. An anti-pan GSK3 beta antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and GSK3 beta present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho GSK3 beta (S9) antibody is added to detect phosphorylated GSK3 beta. In the remaining wells, rabbit anti-pan-GSK3 beta antibody is used to detect pan GSK3 beta. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GSK3 beta (S9) or pan GSK3 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279815-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279815-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279815-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279815-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279815-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-pan-GSK3 beta detection antibody", "number":"AB279815-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-GSK3 beta (S9)-antibody", "number":"AB279815-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-GSK3 beta Coated Microplate", "number":"AB279815-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279815-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279815-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - NIH/3T3 cell lysate", "number":"AB279815-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogen synthase kinase 3 beta or GSK3 beta is a serine/threonine protein kinase that regulates various cellular processes. It is also referred to as GSK3B and has a molecular weight of approximately 47 kilodaltons (kDa). This enzyme is widely expressed in many tissues with notably high expression levels in the brain. Mechanically GSK3 beta phosphorylates target proteins affecting their activity stability and interactions with other proteins.

Biological function summary

As an important regulatory protein GSK3 beta plays a role in multiple cellular functions including metabolism cell cycle and cell signaling. It often interacts with components in signaling complexes and modulates the activity of these complexes involving phosphorylation. It is involved in the regulation of transcription factors such as c-Myc and β-catenin influencing cell fate and survival. This ability to modulate diverse functions highlights GSK3 beta's importance in cellular regulation.

Pathways

GSK3 beta participates in significant signaling pathways such as the Wnt/β-catenin and insulin signaling pathways. In the Wnt pathway GSK3 beta phosphorylates β-catenin marking it for degradation which regulates gene transcription. In insulin signaling it interacts with proteins like AKT to mediate glycogen synthesis. These interactions highlight its role in maintaining cellular homeostasis and energy balance.

GSK3 beta has implications in Alzheimer's disease and type 2 diabetes. It is associated with tau protein hyperphosphorylation and neurofibrillary tangles in Alzheimer's disease. In diabetes its role in insulin signaling impacts glucose metabolism. Further GSK3 beta's dysfunction relates to disrupted signaling related to these conditions positioning it as a potential therapeutic target.

style

left-column

Product protocols

style

left-column

Target data

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

Sandwich ELISA - Phospho-GSK3 beta (S9) and Total GSK3 beta ELISA Kit (AB279815)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise