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AB279818

Phospho-HDAC2 (S394) ELISA Kit

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Phospho-HDAC2 (S394) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-HDAC2 (S394) in Human in Cell/Tissue Extracts samples.

View Alternative Names

Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

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Sandwich ELISA - Phospho-HDAC2 (S394) ELISA Kit (AB279818)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-HDAC2 (S394) ELISA Kit (AB279818)

Positive Control.

HeLa cells were treated with Calyculin A.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-HDAC2 (S394) ELISA Kit (AB279818)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-HDAC2 (S394) ELISA Kit (AB279818)

HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.

Sandwich ELISA - Phospho-HDAC2 (S394) ELISA Kit (AB279818)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-HDAC2 (S394) ELISA Kit (AB279818)

HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-HDAC2 (S394) ELISA Kit (ab279818) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated HDAC2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-HDAC2. An anti-pan HDAC2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HDAC2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-HDAC2 (S394) antibody is used to detect phosphorylated HDAC2. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-HDAC2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279818-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279818-CMP05", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279818-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279818-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279818-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279818-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-HDAC2 Coated Microplate", "number":"AB279818-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-HDAC2 (S394)-antibody", "number":"AB279818-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279818-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279818-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC2 or Histone Deacetylase 2 is an important enzyme that plays a role in chromatin remodeling and gene expression regulation. It removes acetyl groups from histone tails allowing chromatin to condense and silencing gene expression. HDAC2 has a molecular weight of approximately 55 kDa. It is expressed in various tissues including brain heart and skeletal muscles indicating its importance in different physiological areas. HDAC2 proteins are often studied using methods like HDAC2 ELISA and HDAC2 anticuero in research settings.
Biological function summary

HDAC2 is involved in cell cycle regulation and differentiation. It is part of the co-repressor complex and interacts with other proteins such as Sin3 and NuRD to repress transcription. These interactions are vital for maintaining normal cellular functions and ensuring proper response to external signals. This protein is also connected to HEK293T cells which are a common model in scientific studies due to their robust expression of proteins like HDAC2.

Pathways

HDAC2 participates in the regulation of transcriptional activity and cellular stress response. It is an important player in the histone modification pathway interacting with proteins such as HDAC1 and transcriptional regulators like REST. HDAC2 is also linked with the Notch signaling pathway which is essential for cell fate decisions. These pathways highlight its role in both maintaining cellular homeostasis and adapting to changes in the cellular environment.

HDAC2 has a significant connection to neurodegenerative diseases and various cancers. Alzheimer's disease for instance shows altered expression levels of HDAC2 affecting synaptic plasticity and memory formation. In cancer HDAC2 interacts with oncogenic proteins such as p53 influencing tumor progression and metastasis. Monitoring HDAC2 levels and activity could provide insights into disease mechanisms and potential therapeutic targets.
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Product protocols

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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR (PubMed : 12724404). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed : 37137925). Also deacetylates non-histone targets : deacetylates TSHZ3, thereby regulating its transcriptional repressor activity (PubMed : 19343227). May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation (By similarity). Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A (PubMed : 21965678). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 35044827).
See full target information HDAC2 phospho S394
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