Phospho-IKB alpha (S32) and Total IKB alpha ELISA Kit

Product details

Phospho-IKB alpha (S32) and Total IKB alpha ELISA Kit (ab279828) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated IKB alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-IKB alpha (S32) and total IKB alpha. An anti-pan IKB alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and IKB alpha present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho IKB alpha (S32) antibody is added to detect phosphorylated IKB alpha. In the remaining wells, biotinylated anti-pan-IKB alpha antibody is used to detect pan IKB alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IKB alpha (S32) or pan IKB alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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