Phospho-Insulin Receptor (Y1189) and Total Insulin Receptor ELISA Kit

Product details

Phospho-Insulin Receptor (Y1189) and Total Insulin Receptor ELISA Kit (ab279831) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Insulin Receptor protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Insulin Receptor (Y1189) and total Insulin Receptor. An anti-pan Insulin Receptor antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Insulin Receptor present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Insulin Receptor (Y1189) antibody is added to detect phosphorylated Insulin Receptor. In the remaining wells, biotinylated anti-pan-Insulin Receptor antibody is used to detect pan Insulin Receptor. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP- conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin Receptor (Y1189) or pan Insulin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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