AB279841

Phospho-JNK1 (T183/Y185) ELISA Kit

Name: Phospho-JNK1 (T183/Y185) ELISA Kit (ab279841) | Abcam
Brand: Abcam Limited
SKU: AB279841
Price: 1035 USD
Availability: InStock

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Phospho-JNK1 (T183/Y185) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-JNK1 (T183/Y185) in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

sELISA

Supplier Data

Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

Positive Control.

HepG2 cells were treated with IL-1 beta at 37°C for 30 mins.

Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

HepG2 cells untreated/treated with IL-1 beta.

HepG2 cells were untreated or treated with 25 ng/ml IL-1 beta for 30 mins.

sELISA

Supplier Data

Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

HepG2 cells untreated/treated with IL-1 beta.

HepG2 cells were untreated or treated with 25 ng/ml IL-1 beta for 30 mins.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Phospho-JNK1 (T183/Y185) ELISA Kit (ab279841) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated JNK1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-JNK1. An anti-pan JNK1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and JNK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-JNK1 (T183/Y185) antibody is used to detect phosphorylated JNK1. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of JNK1 (T183/Y185) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279841-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279841-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279841-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-JNK1 (T183/Y185)-antibody", "number":"AB279841-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279841-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279841-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - HepG2 cell lysate", "number":"AB279841-CMP01", "productcode":"" }, { "size":"1 x 25 µL", "name":"2000x HRP-conjugated anti-rabbit IgG concentrate", "number":"AB279841-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279841-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-JNK1 Coated Microplate", "number":"AB279841-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 also known as c-Jun N-terminal kinase 1 is a member of the mitogen-activated protein kinase (MAPK) family with a molecular weight of approximately 46 kDa. It is expressed in various tissues throughout the body including the brain heart liver and skeletal muscle. JNK1 exists in multiple isoforms due to alternative splicing. The JNK1 protein is activated by dual phosphorylation on threonine and tyrosine residues a process integral to its function as a stress-activated protein kinase. Commonly used research tools include JNK antibodies and phospho JNK antibodies which help detect the activated forms of this kinase during cellular studies.

Biological function summary

This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.

Pathways

The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.

JNK1 has connections to disorders such as cancer and neurodegenerative diseases. Abnormal activation of JNK1 can lead to irregular cell proliferation making it pertinent in cancer. Similarly in neurodegenerative diseases like Alzheimer's JNK1's involvement in neuronal apoptosis turns critical. It regulates tau phosphorylation connecting JNK1 with tauopathies observed in Alzheimer's disease. Researchers often investigate these associations to understand the mechanisms that underpin these conditions and develop targeted therapies.

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Product protocols

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Target data

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Phospho-JNK1 (T183/Y185) ELISA Kit

Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

Sandwich ELISA - Phospho-JNK1 (T183/Y185) ELISA Kit (AB279841)

Key facts

Detection method

Sample types

Reacts with

Assay type

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Product promise