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AB279852

Phospho-KDM1/LSD1 (S112) ELISA Kit

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Phospho-KDM1/LSD1 (S112) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-KDM1/LSD1 (S112) in Human in Cell/Tissue Extracts samples.

View Alternative Names

AOF2, KDM1, KIAA0601, LSD1, KDM1A, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

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Sandwich ELISA - Phospho-KDM1/LSD1 (S112) ELISA Kit (AB279852)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-KDM1/LSD1 (S112) ELISA Kit (AB279852)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated with Calyculin A and Pervanadate.

Sandwich ELISA - Phospho-KDM1/LSD1 (S112) ELISA Kit (AB279852)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-KDM1/LSD1 (S112) ELISA Kit (AB279852)

Positive Control.

Jurkat cells were treated with Calyculin A and Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-KDM1/LSD1 (S112) ELISA Kit (AB279852)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-KDM1/LSD1 (S112) ELISA Kit (AB279852)

Jurkat cells were treated with/without Calyculin A and Pervanadate. Jurkat cells were treated with Calyculin A and Pervanadate.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-KDM1/LSD1 (S112) ELISA Kit (ab279852) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated KDM1/LSD1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-KDM1/LSD1. An anti-pan KDM1/LSD1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and KDM1/LSD1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-KDM1/LSD1 (S112) antibody is used to detect phosphorylated KDM1/LSD1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of KDM1/LSD1 (S112) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279852-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279852-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279852-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279852-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279852-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-KDM1/LSD1 Coated Microplate", "number":"AB279852-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-KDM1/LSD1 (S112)-antibody", "number":"AB279852-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279852-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279852-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated Jurkat cell lysate", "number":"AB279852-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed : 15620353, PubMed : 15811342, PubMed : 16079794, PubMed : 16079795, PubMed : 16140033, PubMed : 16223729, PubMed : 27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed : 15620353, PubMed : 15811342, PubMed : 16079794, PubMed : 21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed : 15620353, PubMed : 20389281, PubMed : 21300290, PubMed : 23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed : 16079794, PubMed : 16140033, PubMed : 16885027, PubMed : 21300290, PubMed : 23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed : 16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed : 29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed : 29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed : 16079794, PubMed : 16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed : 20562920, PubMed : 27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed : 20389281).
See full target information KDM1A phospho S112
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