Phospho-LKB1 (Ser428) and Total LKB1 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-LKB1 (Ser428) and Total LKB1 in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-LKB1 (Ser428) and Total LKB1 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-LKB1 (Ser428) and Total LKB1 in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-LKB1 (S428) and Total LKB1 ELISA Kit (ab279851) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated LKB1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-LKB1 (S428) and total LKB1. An anti-pan LKB1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and LKB1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho LKB1 (S428) antibody is added to detect phosphorylated LKB1. In the remaining wells, biotinylated anti-pan-LKB1 antibody is used to detect pan LKB1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LKB1 (S428) or pan LKB1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LKB1 also known as STK11 is a serine/threonine kinase with a molecular weight of approximately 48 kDa. This protein is widely expressed in various tissues including the liver pancreas and skeletal muscle. LKB1 plays an essential role in cellular energy homeostasis by phosphorylating and activating a group of at least 14 different proteins within the AMP-activated protein kinase (AMPK) family. The LKB1 protein acts as a master upstream kinase ensuring proper cellular energy balance and metabolism under conditions of nutrient stress.
- Biological function summary
LKB1 is involved in controlling cell growth polarity and metabolism. It forms a complex with STRAD and MO25 which are necessary for its activation and function. By influencing these biological processes LKB1 regulates cell cycle arrest in response to stress signals and contributes to maintaining cellular integrity. The regulation of these processes positions LKB1 as an important factor in managing how cells respond to metabolic challenges.
- Pathways
Its function integrates into major signaling networks like the AMPK and mTOR pathways. LKB1 activates AMPK which in turn affects mTOR signaling a pathway critical for cell growth and autophagy regulation. LKB1's interaction with AMPK links cellular energy levels to the control of biosynthetic pathways and cell cycle progression. The LKB1-AMPK-mTOR axis for instance highlights the protein's role in managing how cells balance growth-related activities and energy conservation.
- Associated diseases and disorders
LKB1 mutations or malfunctions are linked to Peutz-Jeghers syndrome and various forms of cancer such as lung cancer. Altered LKB1 activity affects its ability to suppress tumors primarily through its pathway interactions with AMPK and mTOR. Dysfunction in this pathway leads to unregulated cell growth and subsequently tumorigenesis. Understanding LKB1's role in these conditions aids in developing targeted therapies and diagnostic tools to manage associated risks and interventions effectively.
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2 product images
Sandwich ELISA - Phospho-LKB1 (Ser428) and Total LKB1 ELISA Kit (ab279851)
Positive Control.
COS cells were treated with Forskolin.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-LKB1 (Ser428) and Total LKB1 ELISA Kit (ab279851)
Forskolin treated COS cells treated with/without LPP.
COS cells were treated with Forskolin. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.
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