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AB279856

Phospho-MARCKS (S152/156) ELISA Kit

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Phospho-MARCKS (S152/156) ELISA Kit is a ELISA for the measurement of Phospho-MARCKS (S152/156) in Human in Cell/Tissue Extracts samples.

View Alternative Names

MACS, PRKCSL, MARCKS, Myristoylated alanine-rich C-kinase substrate, 80K-L protein, PKCSL

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Sandwich ELISA - Phospho-MARCKS (S152/156) ELISA Kit (AB279856)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-MARCKS (S152/156) ELISA Kit (AB279856)

Positive Control.

HeLa cells were treated with TPA.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-MARCKS (S152/156) ELISA Kit (AB279856)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-MARCKS (S152/156) ELISA Kit (AB279856)

COS cells untreated/treated with TPA. COS cells were untreated or treated with TPA.

Sandwich ELISA - Phospho-MARCKS (S152/156) ELISA Kit (AB279856)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-MARCKS (S152/156) ELISA Kit (AB279856)

COS cells untreated/treated with TPA. COS cells were untreated or treated with TPA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-MARCKS (S152/156) ELISA Kit (ab279856) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated MARCKS protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-MARCKS. An anti-pan MARCKS antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MARCKS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-MARCKS (S152/156) antibody is used to detect phosphorylated MARCKS. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MARCKS (S152/156) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes (PubMed : 23704996, PubMed : 36009319). Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis (PubMed : 23704996). During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages (PubMed : 37949888). Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type. Participates in the regulation of neurite initiation and outgrowth by interacting with components of cellular machinery including CDC42 that regulates cell shape and process extension through modulation of the cytoskeleton (By similarity). Plays also a role in axon development by mediating docking and fusion of RAB10-positive vesicles with the plasma membrane (By similarity).
See full target information MARCKS phospho S152
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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