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AB279860

Phospho-MEK3 (S189) ELISA Kit

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Phospho-MEK3 (S189) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-MEK3 (S189) in Human in Cell/Tissue Extracts samples.

View Alternative Names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2

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Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)

Positive Control.

HeLa cells were treated with UV.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)

HeLa cells treated with/without UV. HeLa cells were untreated or treated with UV.

Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)

HeLa cells treated with/without UV. HeLa cells were untreated or treated with UV.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-MEK3 (S189) ELISA Kit (ab279860) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated MEK3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-MEK3. An anti-pan MEK3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MEK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-MEK3 (S189) antibody is used to detect phosphorylated MEK3. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MEK3 (S189) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279860-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279860-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279860-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279860-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279860-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-MEK3 (S189)-antibody", "number":"AB279860-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-MEK3 Coated Microplate", "number":"AB279860-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279860-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279860-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279860-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 is a kinase that plays a significant mechanical role within cells. It is part of the MAP kinase family sharing functions with enzymes like MEK6. MEK3 has a molecular mass of approximately 38 kDa and is localized in cytoplasmic regions of various cell types. This protein is expressed in a wide range of tissues indicating its diverse functional roles across different biological systems.
Biological function summary

MEK3 is involved in the phosphorylation cascade within the mitogen-activated protein kinase (MAPK) pathway. It often exists as part of a complex working alongside other kinases to activate p38 MAPK which then regulates gene expression. The involvement of MEK3 in this signaling cascade influences processes such as cellular stress response and inflammation. Its ability to modulate these processes makes MEK3 an important component in maintaining cellular homeostasis.

Pathways

MEK3 functions as an essential link within the stress-activated pathways like the MAPK signaling pathway. It phosphorylates and activates the p38 MAPK pathway therefore regulating activities related to cellular stress and inflammatory responses. MEK3 works in conjunction with related kinases such as MEK6 to propagate the MAPK signaling cascade further emphasizing its important role in these pathways.

MEK3 has a documented involvement in inflammatory conditions and cancer. Its role in activating the p38 MAPK pathway connects it to inflammatory diseases where dysregulated signaling may lead to excessive inflammation. Furthermore aberrant MEK3 activity is associated with certain cancers as it affects cell proliferation and survival. MEK3's interactions with other kinases like MEK6 underline its involvement in these pathological processes making it a potential target for therapeutic intervention.
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Product protocols

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Target data

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
See full target information MAP2K3 phospho S189
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