Phospho-MSK1 (S376) and Total MSK1 ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-MSK1 (S376) and Total MSK1 ELISA Kit (AB279865)
Positive Control.
HeLa cells were treated with Calyculin A.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-MSK1 (S376) and Total MSK1 ELISA Kit (AB279865)
HeLa cells treated with/without Calyculin A. HeLa cells were untreated or treated with Calyculin A.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-MSK1 (S376) and Total MSK1 ELISA Kit (AB279865)
HeLa cells treated with/without Calyculin A. HeLa cells were untreated or treated with Calyculin A.
Reactivity data
Product details
Phospho-MSK1 (S376) and Total MSK1 ELISA Kit (ab279865) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated MSK1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-MSK1 and total MSK1. An anti-pan MSK1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MSK1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho MSK1 (S376) antibody is added to detect phosphorylated MSK1. In the remaining wells, mouse anti-pan-MSK1 antibody is used to detect pan MSK1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MSK1 (S376) or pan MSK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MSK1 influences cell proliferation and survival. It operates as part of the MAPK signaling pathway driven by extracellular signals. MSK1 activation occurs through ERK1/2 and p38 MAPK therefore linking it to essential cellular processes. As a kinase MSK1 phosphorylates downstream targets contributing to inflammatory responses apoptosis and gene expression modulation. MSK1 often partners with other proteins to form complexes enhancing its regulatory capabilities.
Pathways
MSK1 plays a role in both the MAPK and NF-kB pathways. These pathways contribute to controlling cell growth and immune responses. In the MAPK pathway the involvement of MSK1 influences further regulation of transcription factors. In connection with the NF-kB pathway MSK1 can regulate NF-kB activity promoting gene expression related to inflammation. Related proteins in these pathways include ERK1/2 which activate MSK1 enhancing the kinase's function in these critical cellular circuits.
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