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AB279878

Phospho-p53 (S15) and Total p53 ELISA Kit

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Phospho-p53 (S15) and Total p53 ELISA Kit is a ELISA for the measurement of Phospho-p53 (S15) and Total p53 in Human in Cell/Tissue Extracts samples.

View Alternative Names

P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, P53, TP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

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Sandwich ELISA - Phospho-p53 (S15) and Total p53 ELISA Kit (AB279878)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-p53 (S15) and Total p53 ELISA Kit (AB279878)

T47D cells treated/untreated with UV.

T47D cells were untreated or exposed to 50 J/m2 of UV light followed by a 4 hours recovery period before lysis.

Sandwich ELISA - Phospho-p53 (S15) and Total p53 ELISA Kit (AB279878)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-p53 (S15) and Total p53 ELISA Kit (AB279878)

Positive Control.

T47D cells were exposed to 50 J/m2 of UV light followed by a 4 hours recovery period.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-p53 (S15) and Total p53 ELISA Kit (AB279878)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-p53 (S15) and Total p53 ELISA Kit (AB279878)

T47D cells treated/untreated with UV.

T47D cells were untreated or exposed to 50 J/m2 of UV light followed by a 4 hours recovery period before lysis.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Phospho-p53 (S15) and Total p53 ELISA Kit (ab279878) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated p53 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human p53 (Ser15) and pan p53. The left side of a 96-well plate is coated with anti-p53 (Ser15) (distinguished by red marker) and the right side of the plate is coated with anti-pan p53 antibody (distinguished by black marker). Samples are pipetted into the wells and phosphorylated p53 (left side) and pan p53 (right side) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pan-p53 is used to detect phosphorylated p53 (Ser15) or pan p53. After washing away unbound antibody, HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of p53 (Ser15) or pan p53 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279878-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279878-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - T47D cell lysate", "number":"AB279878-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-p53-antibody", "number":"AB279878-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279878-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279878-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279878-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279878-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279878-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Phospho and non-phospho p53 Coated Microplate", "number":"AB279878-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
Biological function summary

P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.

Pathways

P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.

P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
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Product protocols

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Target data

Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 35618207, PubMed : 36634798, PubMed : 38653238, PubMed : 9840937). Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17189187, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 38653238, PubMed : 9840937). Negatively regulates cell division by controlling expression of a set of genes required for this process (PubMed : 11025664, PubMed : 12524540, PubMed : 12810724, PubMed : 15186775, PubMed : 15340061, PubMed : 17317671, PubMed : 17349958, PubMed : 19556538, PubMed : 20673990, PubMed : 20959462, PubMed : 22726440, PubMed : 24051492, PubMed : 24652652, PubMed : 9840937). One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed : 12524540, PubMed : 17189187). Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed : 12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed : 12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed : 24051492).
See full target information TP53

Additional targets

TP53 phospho S15
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