JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279871

Phospho-p95/NBS1 (S343) ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Phospho-p95/NBS1 (S343) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-p95/NBS1 (S343) in Human in Cell/Tissue Extracts samples.

View Alternative Names

NBS, NBS1, P95, NBN, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, hNbs1

3 Images
Sandwich ELISA - Phospho-p95/NBS1 (S343) ELISA Kit (AB279871)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-p95/NBS1 (S343) ELISA Kit (AB279871)

Positive Control.

T47D cells were irradiated with UV.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-p95/NBS1 (S343) ELISA Kit (AB279871)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-p95/NBS1 (S343) ELISA Kit (AB279871)

T47D cells treated/untreated with UV. T47D cells were untreated or treated with UV.

Sandwich ELISA - Phospho-p95/NBS1 (S343) ELISA Kit (AB279871)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-p95/NBS1 (S343) ELISA Kit (AB279871)

T47D cells treated/untreated with UV. T47D cells were untreated or treated with UV.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phospho-p95/NBS1 (S343) ELISA Kit (ab279871) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated p95/NBS1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-p95/NBS1. An anti-pan p95/NBS1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and p95/NBS1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-p95/NBS1 (S343) antibody is used to detect phosphorylated p95/NBS1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p95/NBS1 (S343) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279871-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279871-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279871-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - T47D cell lysate", "number":"AB279871-CMP02", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279871-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-p95/NBS1 (S343)-antibody", "number":"AB279871-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279871-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279871-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279871-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-p95/NBS1 Coated Microplate", "number":"AB279871-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target known as p95/NBS1 also referred to as NBN protein is a critical component in the DNA damage response mechanism. The full molecular weight of the NBS1 protein is approximately 95 kDa. This protein is abundantly expressed in various tissues particularly where there is a higher rate of cell division or repair such as in the thymus and testis. It plays an important role in maintaining the stability of the genome through its involvement in the repair of double-strand breaks.
Biological function summary

The NBS1 protein functions as an essential component of the MRN complex which also includes MRE11 and RAD50 proteins. This complex is fundamental in accurately detecting DNA double-strand breaks and initiating repair processes. Through its actions NBS1 facilitates numerous cellular processes that preserve genomic integrity such as homologous recombination and non-homologous end joining. Its influence in controlling the cell cycle further emphasizes its role in maintaining cellular health.

Pathways

The NBS1 protein engages significantly in the DNA damage response and repair pathways notably impacting the ATM signaling pathway. By interacting with proteins such as ATM kinase NBS1 facilitates the phosphorylation and activation of several downstream effectors necessary for DNA repair and cell cycle checkpoints. The MRN complex also links with the RAD50 protein highlighting its involvement in these pathways.

Mutations in the NBS1 gene are linked to Nijmegen breakage syndrome characterized by sensitivity to radiation immune deficiency and increased cancer risk. Additionally NBS1's role in pathways makes it pertinent to certain cancer pathologies where its malfunction can contribute to genomic instability. The NBN protein through its interaction with ATM and RAD50 assists in understanding its connection with predispositions to these diseases.
style
left-column

Product protocols

style
left-column

Target data

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed : 10888888, PubMed : 15616588, PubMed : 18411307, PubMed : 18583988, PubMed : 18678890, PubMed : 19759395, PubMed : 23115235, PubMed : 28216226, PubMed : 28867292, PubMed : 9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed : 19759395, PubMed : 28867292, PubMed : 9705271). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed : 19759395, PubMed : 9705271). The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (PubMed : 19759395, PubMed : 28867292, PubMed : 9705271). Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites (PubMed : 12419185, PubMed : 15616588, PubMed : 18411307, PubMed : 18582474, PubMed : 18583988, PubMed : 18678890, PubMed : 19759395, PubMed : 19804756, PubMed : 23762398, PubMed : 24534091, PubMed : 27814491, PubMed : 27889449, PubMed : 33836577). Recruits MRE11 and RAD50 components of the MRN complex to DSBs in response to DNA damage (PubMed : 12419185, PubMed : 18411307, PubMed : 18583988, PubMed : 18678890, PubMed : 24534091, PubMed : 26438602). Promotes the recruitment of PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites, activating their functions (PubMed : 15064416, PubMed : 15616588, PubMed : 15790808, PubMed : 16622404, PubMed : 22464731, PubMed : 30952868, PubMed : 35076389). Mediates the recruitment of phosphorylated RBBP8/CtIP to DSBs, leading to cooperation between the MRN complex and RBBP8/CtIP to initiate end resection (PubMed : 19759395, PubMed : 27814491, PubMed : 27889449, PubMed : 33836577). RBBP8/CtIP specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts (PubMed : 27814491, PubMed : 27889449, PubMed : 30787182, PubMed : 33836577). The MRN complex is also required for the processing of R-loops (PubMed : 31537797). NBN also functions in telomere length maintenance via its interaction with TERF2 : interaction with TERF2 during G1 phase preventing recruitment of DCLRE1B/Apollo to telomeres (PubMed : 10888888, PubMed : 28216226). NBN also promotes DNA repair choice at dysfunctional telomeres : NBN phosphorylation by CK2 promotes non-homologous end joining repair at telomeres, while unphosphorylated NBN promotes microhomology-mediated end-joining (MMEJ) repair (PubMed : 28216226). Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex (PubMed : 23762398).
See full target information NBN phospho S343
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB279877

Phospho-p53 (S15) ELISA Kit

Sandwich ELISA - Phospho-p53 (S15) ELISA Kit (AB279877)

  • 0
  • 0
Assay Kits

AB279939

Phospho-STAT2 (Y689) ELISA Kit

Sandwich ELISA - Phospho-STAT2 (Y689) ELISA Kit (AB279939)

  • 0
  • 0
Primary Antibodies

AB109453

Anti-p95/NBS1 (phospho S343) antibody [EP178]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-p95/NBS1 (phospho S343) antibody [EP178] (AB109453)

  • 0
  • 0
Assay Kits

AB176662

JNK 1/2 (pT183/Y185 + Total) ELISA Kit

SimpleStep

ELISA - JNK 1/2 (pT183/Y185 + Total) ELISA Kit (AB176662)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com