Phospho-PAK2 (S20) ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-PAK2 (S20) in Human in Cell Lysate samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation (PubMed:12853446, PubMed:16617111, PubMed:19273597, PubMed:19923322, PubMed:33693784, PubMed:7744004, PubMed:9171063). Acts as a downstream effector of the small GTPases CDC42 and RAC1 (PubMed:7744004). Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues (PubMed:7744004). Full-length PAK2 stimulates cell survival and cell growth (PubMed:7744004). Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration (PubMed:21317288). Phosphorylates JUN and plays an important role in EGF-induced cell proliferation (PubMed:21177766). Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP (PubMed:21724829). Phosphorylates CASP7, thereby preventing its activity (PubMed:21555521, PubMed:27889207). Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis (PubMed:19273597, PubMed:19923322). On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway (PubMed:12853446, PubMed:16617111, PubMed:9171063). Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (PubMed:15234964).
Alternative names
Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, PAK-2, PAK2
What's included?
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Phospho-PAK2 (S20) ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-PAK2 (S20) in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-PAK2 (S20) ELISA Kit (ab279882) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PAK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-PAK2. An anti-pan PAK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and PAK2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-PAK2 (S20) antibody is used to detect phosphorylated PAK2. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PAK2 (S20) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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3 product images
Sandwich ELISA - Phospho-PAK2 (S20) ELISA Kit (ab279882)
Positive Control.
HeLa cells were treated with Calyculin A.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-PAK2 (S20) ELISA Kit (ab279882)
HeLa cells treated with/without Calyculin A.
HeLa cells were treated with Calyculin A. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Sandwich ELISA - Phospho-PAK2 (S20) ELISA Kit (ab279882)
HeLa cells treated with/without Calyculin A.
HeLa cells were treated with Calyculin A. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
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