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AB279886

Phospho-PDGFR beta (Y751) ELISA Kit

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Phospho-PDGFR beta (Y751) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-PDGFR beta (Y751) in Human in Cell/Tissue Extracts samples.

View Alternative Names

CD140b, PDGFR, PDGFR1, PDGFRB, Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1

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Sandwich ELISA - Phospho-PDGFR beta (Y751) ELISA Kit (AB279886)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-PDGFR beta (Y751) ELISA Kit (AB279886)

Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with PV.

Sandwich ELISA - Phospho-PDGFR beta (Y751) ELISA Kit (AB279886)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-PDGFR beta (Y751) ELISA Kit (AB279886)

Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with PV.

Sandwich ELISA - Phospho-PDGFR beta (Y751) ELISA Kit (AB279886)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-PDGFR beta (Y751) ELISA Kit (AB279886)

Positive Control.

Jurkat cells were treated with PV (Pervanadate).

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-PDGFR beta (Y751) ELISA Kit (ab279886) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PDGFR beta protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-PDGFR beta. An anti-pan PDGFR beta antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and PDGFR beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and rabbit anti-phospho-PDGFR beta (Y751) antibody is used to detect phosphorylated PDGFR beta. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGFR beta (Y751) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279886-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279886-CMP06", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279886-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279886-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279886-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279886-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-PDGFR beta (Y751)-antibody", "number":"AB279886-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-PDGFR beta Coated Microplate", "number":"AB279886-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279886-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279886-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.
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Product protocols

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Target data

The protein expressed by the PDGFRB gene is a tyrosine-protein kinase that functions as a cell-surface receptor for various PDGF homodimers and heterodimers, playing an essential role in embryonic development, cell proliferation, survival, differentiation, chemotaxis, and migration. It is crucial for blood vessel development by promoting the proliferation and migration of, as well as recruitment of, pericytes and smooth muscle cells to endothelial cells. It has a role in vascular smooth muscle cell migration and neointima formation at injury sites, and is necessary for normal cardiovascular development and pericyte recruitment in kidney glomeruli. The protein facilitates actin cytoskeleton rearrangement and membrane ruffle formation. Ligand binding activates signaling cascades depending on the ligand bound and interactions with PDGFRA, leading to phosphorylation of several substrates, activation of signaling pathways such as AKT1, MAPK, and SRC family kinases, and production of molecules like diacylglycerol and inositol trisphosphate. Receptor signaling is downregulated by protein phosphatases and receptor internalization. This supplementary information is collated from multiple sources and compiled automatically.
See full target information PDGFRB phospho Y751
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websiteProtocolBooklet
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