Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-PYK2 (Y402) and Total PYK2 in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-PYK2 (Y402) and Total PYK2 in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit (ab279902) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PYK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-PYK2 and total PYK2. An anti-pan PYK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and PYK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, mouse anti-phospho PYK2 (Y402) antibody is added to detect phosphorylated PYK2. In the remaining wells, biotinylated anti-pan-PYK2 antibody is used to detect pan PYK2. After washing away unbound antibody, HRP-conjugated anti-mouse IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PYK2 (Y402) or pan PYK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PYK2 also known as Protein Tyrosine Kinase 2 Beta (PTK2B) is a protein with a mass of approximately 116 kDa. This enzyme belongs to the focal adhesion kinase (FAK) family and functions as a non-receptor protein tyrosine kinase. Mechanically PYK2 is involved in calcium-dependent signaling and is activated by increases in intracellular calcium levels. It plays a critical role in cell proliferation migration and survival. PYK2 expresses widely in neuronal tissues hematopoietic cells and various epithelial cells.
- Biological function summary
PYK2 interacts with integrins and G-protein-coupled receptors (GPCRs) linking extracellular signals to intracellular pathways. It forms complexes with other signaling molecules including Rac1 and c-Src facilitating downstream signaling. These interactions contribute to cellular communication and adhesion processes especially in the central nervous system and immune cells. Through its involvement in signal transduction PYK2 supports cellular responses to environmental changes.
- Pathways
PYK2 participates in the MAPK and JNK signaling pathways. These pathways are critical for transmitting signals from the cell surface to the nucleus influencing cellular growth and stress responses. PYK2 interacts closely with other proteins such as Src and FAK which help coordinate signals that regulate cellular dynamics. Through these pathways PYK2 modulates processes involved in the structural reorganization of cells and tissues.
- Associated diseases and disorders
PYK2 has been linked to neurodegenerative diseases and certain types of cancers. In Alzheimer's disease PYK2 may contribute to synaptic dysfunction and cognitive decline as it interacts with amyloid-beta peptides and tau proteins. In cancer PYK2 overexpression can promote cancer cell invasion metastasis and resistance to apoptosis often in coordination with proteins like FAK. Therefore understanding the role of PYK2 in these conditions provides insights for therapeutic developments.
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3 product images
Sandwich ELISA - Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit (ab279902)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 min.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit (ab279902)
Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with Pervanadate for 10 minutes.
Sandwich ELISA - Phospho-PYK2 (Y402) and Total PYK2 ELISA Kit (ab279902)
Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with Pervanadate for 10 minutes.
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