Phospho-RPS6 (S235/S236) and Total RPS6 ELISA Kit

Product details

Phospho-RPS6 (S235/S236) and Total RPS6 ELISA Kit (ab279913) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated RPS6 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-RPS6 (S235/S236) and total RPS6. An anti-pan RPS6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RPS6 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho RPS6 (S235/S236) antibody is added to detect phosphorylated RPS6 (S235/S236). In the remaining wells, rabbit anti-pan-RPS6 antibody is used to detect pan RPS6. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RPS6 (S235/S236) or pan RPS6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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