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AB279912

Phospho-RPS6 (S235/S236) ELISA Kit

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Phospho-RPS6 (S235/S236) ELISA Kit is a ELISA for the measurement of Phospho-RPS6 (S235/S236) in Mouse in Cell/Tissue Extracts samples.

View Alternative Names

Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33, Rps6

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Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (AB279912)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (AB279912)

NIH/3T3 cells treated with/without PDGF.

NIH/3T3 cells were untreated or treated with PDGFBB for 10 minutes.

Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (AB279912)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (AB279912)

NIH/3T3 cells treated with/without PDGF.

NIH/3T3 cells were untreated or treated with PDGFBB for 10 minutes.

Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (AB279912)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (AB279912)

Positive Control.

NIH/3T3 cells were treated with PDGFBB at 37°C for 10 min.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Mouse

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-RPS6 (S235/S236) ELISA Kit (ab279912) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated RPS6 (S235/S236) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-RPS6. An anti-pan RPS6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RPS6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-RPS6 (S235/S236) antibody is used to detect phosphorylated RPS6. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RPS6 (S235/S236) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279912-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279912-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279912-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-RPS6 (S235/S236)-antibody", "number":"AB279912-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279912-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279912-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-RPS6 Coated Microplate", "number":"AB279912-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - NIH/3T3 cell lysate", "number":"AB279912-CMP02", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279912-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279912-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.
Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.
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Product protocols

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Target data

Component of the 40S small ribosomal subunit. Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed : 36517592). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (By similarity).
See full target information Rps6 phospho S235
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websiteProtocolBooklet
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Product promise

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