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Phospho-RPS6 (S235/S236) ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-RPS6 (S235/S236) in Mouse in Cell Lysate samples.

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Images

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Mouse

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Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
2X Cell lysate buffer
1 x 5 mL
5X Assay Diluent
1 x 15 mL
HRP-conjugated anti-rabbit IgG concentrate (1000X)
1 x 25 µL
Pan-RPS6 Coated Microplate
1 x 1 Unit
Positive Control - NIH/3T3 cell lysate
1 x 1 Vial
Rabbit anti-phospho-RPS6 (S235/S236)-antibody
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Phospho-RPS6 (S235/S236) ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-RPS6 (S235/S236) in Mouse in Cell Lysate samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Mouse

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Phospho-RPS6 (S235/S236) ELISA Kit (ab279912) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated RPS6 (S235/S236) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-RPS6. An anti-pan RPS6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RPS6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-RPS6 (S235/S236) antibody is used to detect phosphorylated RPS6. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RPS6 (S235/S236) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

Biological function summary

RPS6 is integral to the formation of the ribosomal complex. This complex is essential for the translation initiation process. RPS6 controls the translation capacity of ribosomes and functions in growth and development by regulating protein synthesis rates. Alterations in RPS6 activity can impact cellular growth and size due to its role in ribosome biogenesis.

Activity summary

RPS6 also known as Ribosomal Protein S6 is a component of the 40S ribosomal subunit with a molecular weight of approximately 28 kDa. This protein mainly localizes in the cytoplasm where ribosomes are fixed during protein synthesis. The ribosomal machinery uses RPS6 to translate mRNA into polypeptides operating as an essential part of protein biosynthesis. It expresses in various tissues with higher levels in highly proliferative cells which need extensive protein production for growth.

Pathways

RPS6 plays a significant role in the mTOR and PI3K/AKT signaling pathways. These pathways link nutrient availability to protein synthesis influencing cellular growth and metabolism. RPS6 interacts with proteins like S6K1 which is a downstream target in the mTOR pathway to modulate translation and cell growth processes.

Associated diseases and disorders

RPS6 has connections to cancer and metabolic syndromes. Abnormal RPS6 activation can lead to dysregulated cell growth contributing to oncogenesis. In the context of cancer mTOR signaling where RPS6 functions is often upregulated leading to uncontrolled proliferation. Moreover in metabolic disorders the disrupted pathway activity involves other proteins like AKT fostering insulin resistance and metabolic imbalance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (ab279912), expandable thumbnail

    Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (ab279912)

    Positive Control.

    NIH/3T3 cells were treated with PDGFBB at 37°C for 10 min.

    Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

    Serial dilutions of lysates were analyzed in this ELISA.

  • Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (ab279912), expandable thumbnail

    Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (ab279912)

    NIH/3T3 cells treated with/without PDGF.

    NIH/3T3 cells were untreated or treated with PDGFBB for 10 minutes.

  • Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (ab279912), expandable thumbnail

    Sandwich ELISA - Phospho-RPS6 (S235/S236) ELISA Kit (ab279912)

    NIH/3T3 cells treated with/without PDGF.

    NIH/3T3 cells were untreated or treated with PDGFBB for 10 minutes.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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