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AB279915

Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit

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Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-RSK1 p90 (S380) and Total RSK1 p90 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)

A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml EGF for 20 minutes.

Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)

Positive Control.

A431 cells were treated with EGF at 37°C for 20 min.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (AB279915)

A431 cells treated with/without EGF. A431 cells were untreated or treated with 100 ng/ml EGF for 20 minutes.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Phospho-RSK1 p90 (S380) and Total RSK1 p90 ELISA Kit (ab279915) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated RSK1 p90 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-RSK1 p90 and total RSK1 p90. An anti-pan RSK1 p90 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RSK1 p90 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho RSK1 p90 (S380) antibody is added to detect phosphorylated RSK1 p90 (S380). In the remaining wells, biotinylated anti-pan-RSK1 p90 antibody is used to detect pan RSK1 p90. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSK1 p90 (S380) or pan RSK1 p90 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSK1 also known as p90 ribosomal S6 kinase 1 or p90RSK is a serine/threonine kinase with an approximate mass of 90 kDa. It belongs to the family of proteins that integrate signals from various extracellular stimuli. This kinase is expressed in diverse tissues including brain lung liver and skeletal muscle. At the molecular level RSK1 phosphorylates various target proteins that are involved in regulating cellular growth and survival. Its activity is regulated by phosphorylation events that occur at specific sites such as Serine 238 (E238 in some literature) which are critical for its function.
Biological function summary

RSK1 plays a fundamental role in cell proliferation and differentiation in the context of the p90 protein weight. It is an important component of the mitogen-activated protein kinase (MAPK) signaling pathway and forms part of multi-protein complexes that facilitate its activation and function. RSK1 influences various biological processes by phosphorylating transcription factors histone proteins and other kinases thereby regulating gene expression and protein synthesis. These actions highlight its influence in maintaining cellular homeostasis.

Pathways

RSK1 p90 interacts significantly with the MAPK/ERK and PI3K/AKT pathways. It acts downstream of ERK where it receives activating signals that enable it to phosphorylate substrates within the cell. This role supports its interaction with proteins like Akt and mTOR which are critical mediators of cell growth signals. The interplay between these pathways enables cells to respond to growth factors and stress contributing to the regulation of development and metabolism.

RSK1 is implicated in the progression of cancer and neurological disorders. In cancer abnormal RSK1 signaling can lead to unchecked cell growth and survival where it often interfaces with proteins such as C-RAF and BCL-2 to promote oncogenic pathways. In neurological disorders RSK1 influences pathways that regulate neuron survival and plasticity connecting it to proteins like CREB. Understanding RSK1's role in these conditions can provide insight into targeted therapies that may mitigate these diseases.
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Product protocols

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Target data

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websiteProtocolBooklet
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