Phospho-RSK2 (S386) and Total RSK2 ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-RSK2 (S386) and Total RSK2 ELISA Kit (AB279917)
HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-RSK2 (S386) and Total RSK2 ELISA Kit (AB279917)
HeLa cells treated with/without Calyculin A. HeLa cells were treated or untreated with Calyculin A.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-RSK2 (S386) and Total RSK2 ELISA Kit (AB279917)
Positive Control.
HeLa cells were treated with Calyculin A.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Reactivity data
Product details
Phospho-RSK2 (S386) and Total RSK2 ELISA Kit (ab279917) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated RSK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-RSK2 (S386) and total RSK2. An anti-pan RSK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RSK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho RSK2 (S386) antibody is added to detect phosphorylated eIF4EBP1. In the remaining wells, rabbit anti-pan-RSK2 antibody is used to detect pan RSK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSK2 (S386) or pan RSK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RSK2 influences cell proliferation growth and survival by acting as a bridge in signaling cascades downstream of the extracellular signal-regulated kinase (ERK) pathway. This protein is not part of a larger complex but it interacts with other proteins such as ERK1/2 and substrates like ribosomal protein S6. By modulating these interactions RSK2 helps control gene expression related to cellular growth and differentiation functions.
Pathways
Many cellular processes involve RSK2 particularly through the MAPK/ERK signaling and the PI3K/AKT pathways. It plays a significant role in transmitting signals that regulate cellular and metabolic responses linking external growth factors and hormones to appropriate cellular actions. RSK2 interacts closely with proteins like ERK1/2 within these pathways to facilitate its role in linking upstream signals to downstream effects impacting cellular growth and metabolic activity.
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