Phospho-S6K1 (T421/S424) and Total S6K1 ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-S6K1 (T421/S424) and Total S6K1 ELISA Kit (AB279881)
Positive Control.
T47D cells were treated with recombinant human IGF-1 at 37°C for 30 mins.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-S6K1 (T421/S424) and Total S6K1 ELISA Kit (AB279881)
T47D cells treated/untreated with recombinant IGF-1. T47D cells were untreated or treated with 100 ng/ml recombinant human IGF-1 for 30 minutes.
Reactivity data
Product details
Phospho-S6K1 (T421/S424) and Total S6K1 ELISA Kit (ab279881) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated S6K1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-S6K1 and total S6K1. An anti-pan S6K1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and S6K1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho S6K1 (T421/S424) antibody is added to detect phosphorylated S6K1. In the remaining wells, rabbit anti-pan-S6K1 antibody is used to detect pan S6K1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of S6K1 (T421/S424) or pan S6K1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
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Biological function summary
S6K1 functions as a downstream effector of the mammalian target of rapamycin (mTOR) complex specifically mTORC1. S6K1 interacts with several protein complexes enhancing its regulatory capacity in cellular activities. It modulates protein synthesis by phosphorylating substrates involved in translation. By controlling these processes S6K1 aids in cell size regulation and energy metabolism which are critical for maintaining cellular homeostasis and adaptation to nutrient availability.
Pathways
S6K1 plays an essential role in the mTOR signaling pathway a central regulator of cell growth and metabolism. S6K1 functions in close connection with proteins such as mTOR and Raptor within this pathway. Additionally it is involved in the insulin signaling pathway where it works with proteins like insulin receptor substrate (IRS). Both pathways highlight S6K1's role in nutrient sensing and response emphasizing its significance in energy and protein homeostasis.
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