Phospho-SHC (Y427) and Total SHC ELISA Kit
Be the first to review this product! Submit a review
|
(0 Publication)
- sELISA
Supplier Data
Sandwich ELISA - Phospho-SHC (Y427) and Total SHC ELISA Kit (AB279920)
A431 cells treated with/without EGF. A431 cells were treated or untreated with EGF.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-SHC (Y427) and Total SHC ELISA Kit (AB279920)
Positive Control.
A431 cells were treated with EGF.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-SHC (Y427) and Total SHC ELISA Kit (AB279920)
A431 cells treated with/without EGF. A431 cells were treated or untreated with EGF.
Reactivity data
Product details
Phospho-SHC (Y427) and Total SHC ELISA Kit (ab279920) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SHC protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SHC and total SHC. An anti-pan SHC antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SHC present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho SHC (Y427) antibody is added to detect phosphorylated SHC. In the remaining wells, rabbit anti-pan-SHC antibody is used to detect pan SHC. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SHC (Y427) or pan SHC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SHC protein transmits signals from cell surface receptors to downstream effectors. It does so by linking activated receptors to the Ras-MAPK signaling cascade. SHC often part of larger protein complexes interacts with proteins such as Grb2 and SOS to relay signals necessary for cell growth and differentiation. This interaction leads to the activation of Ras and subsequent mitogen-activated protein kinase pathways influencing various cellular responses.
Pathways
SHC is a central component of the MAPK/ERK and PI3K/AKT pathways which are important for cell growth and survival. In these pathways SHC mediates signals from receptor tyrosine kinases like the epidermal growth factor receptor (EGFR). By interacting with EGFR and other receptors SHC helps propagate signals essential for cellular proliferation and survival. SHC associates with proteins like Grb2 and SOS linking RTKs to the Ras signaling cascade.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
![Western blot - Anti-SHC antibody [EP332Y] (AB33770)](https://content.abcam.com/products/images/shc-antibody-ep332y-ab33770--western-blot-img18793.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com