Phospho-SHIP-1 (Y1020) and Total SHIP-1 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-SHIP-1 (Y1020) and Total SHIP-1 in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-SHIP-1 (Y1020) and Total SHIP-1 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-SHIP-1 (Y1020) and Total SHIP-1 in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-SHIP-1 (Y1020) and Total SHIP-1 ELISA Kit (ab279922) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SHIP-1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SHIP-1 and total SHIP-1. An anti-pan SHIP-1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SHIP-1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho SHIP-1 (Y1020) antibody is added to detect phosphorylated SHIP-1. In the remaining wells, rabbit anti-pan-SHIP-1 antibody is used to detect pan SHIP-1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SHIP-1 (Y1020) or pan SHIP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHIP-1 also known as SHIP or INPP5D is a protein with a molecular mass of approximately 145 kDa. It functions as an inositol polyphosphate-5-phosphatase. This enzyme specifically hydrolyzes the 5’ phosphate from phosphatidylinositol-345-trisphosphate (PIP3) converting it to phosphatidylinositol-34-bisphosphate (PI(34)P2). SHIP-1 is mainly expressed in hematopoietic cells including those of the immune system like B cells T cells and myeloid cells.
- Biological function summary
SHIP-1 plays significant roles in regulating immune cell signaling. It partakes in control over cell growth apoptosis and cellular responses to external signals. SHIP-1 influences the balance between cell survival and programmed cell death by modulating the PI3K signaling pathway which is pivotal for lymphocyte activation and immune functions. SHIP-1 can operate independently or as part of larger signaling complexes with other molecules to ensure immune cells respond appropriately to stimuli.
- Pathways
SHIP-1 plays an integral role in the phosphoinositide signaling pathway and is linked to the PI3K-Akt pathway. It acts as an important regulatory node by limiting the accumulation of PIP3 a necessary component for Akt activation. This regulation allows for controlled signal propagation that influences immune cell activity and development. SHIP-1 also interfaces with proteins like PTEN and SHP-1 which similarly modulate downstream signaling events within these pathways ensuring the maintenance of immune equilibrium.
- Associated diseases and disorders
SHIP-1 is associated with conditions like leukemia and autoimmune diseases. Alterations or deficiencies in SHIP-1 function can lead to abnormal immune responses or uncontrolled cell proliferation. For instance its reduced activity has connections to leukemia where there is excessive cell growth due to unchecked PI3K signaling. Moreover SHIP-1 is associated with autoimmune disorders through interactions with other signaling proteins that might affect the intensity and duration of immune responses highlighting its role in maintaining immune tolerance and preventing self-reactivity.
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3 product images
Sandwich ELISA - Phospho-SHIP-1 (Y1020) and Total SHIP-1 ELISA Kit (ab279922)
Positive Control.
THP1 cells were treated with Pervanadate.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-SHIP-1 (Y1020) and Total SHIP-1 ELISA Kit (ab279922)
THP-1 cells untreated/treated with Pervanadate. THP1 cells were treated with Pervanadate.
Sandwich ELISA - Phospho-SHIP-1 (Y1020) and Total SHIP-1 ELISA Kit (ab279922)
THP-1 cells untreated/treated with Pervanadate. THP1 cells were treated with Pervanadate.
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