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AB279923

Phospho-SHP-1 (S591) ELISA Kit

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Phospho-SHP-1 (S591) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-SHP-1 (S591) in Human in Cell/Tissue Extracts samples.

View Alternative Names

SHIP, SHIP1, INPP5D, Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, SHIP-1, hp51CN

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Sandwich ELISA - Phospho-SHP-1 (S591) ELISA Kit (AB279923)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-SHP-1 (S591) ELISA Kit (AB279923)

Jurkat cells were treated with/without Calyculin A and Pervanadate.

Jurkat cells were treated with Calyculin A and PV.

Sandwich ELISA - Phospho-SHP-1 (S591) ELISA Kit (AB279923)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-SHP-1 (S591) ELISA Kit (AB279923)

Jurkat cells were treated with/without Calyculin A and Pervanadate.

Jurkat cells were treated with Calyculin A and PV.

Sandwich ELISA - Phospho-SHP-1 (S591) ELISA Kit (AB279923)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-SHP-1 (S591) ELISA Kit (AB279923)

Positive Control.

Jurkat cells were treated with Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-SHP-1 (S591) ELISA Kit (ab279923) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SHP-1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SHP-1. An anti-pan SHP-1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SHP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-SHP-1 (S591) antibody is used to detect phosphorylated SHP-1. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SHP-1 (S591) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279923-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279923-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279923-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279923-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-SHP-1 Coated Microplate", "number":"AB279923-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279923-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated Jurkat cell lysate", "number":"AB279923-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279923-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-SHP-1 (S591)-antibody", "number":"AB279923-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279923-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHIP-1 also known as SHIP or INPP5D is a protein with a molecular mass of approximately 145 kDa. It functions as an inositol polyphosphate-5-phosphatase. This enzyme specifically hydrolyzes the 5’ phosphate from phosphatidylinositol-345-trisphosphate (PIP3) converting it to phosphatidylinositol-34-bisphosphate (PI(34)P2). SHIP-1 is mainly expressed in hematopoietic cells including those of the immune system like B cells T cells and myeloid cells.
Biological function summary

SHIP-1 plays significant roles in regulating immune cell signaling. It partakes in control over cell growth apoptosis and cellular responses to external signals. SHIP-1 influences the balance between cell survival and programmed cell death by modulating the PI3K signaling pathway which is pivotal for lymphocyte activation and immune functions. SHIP-1 can operate independently or as part of larger signaling complexes with other molecules to ensure immune cells respond appropriately to stimuli.

Pathways

SHIP-1 plays an integral role in the phosphoinositide signaling pathway and is linked to the PI3K-Akt pathway. It acts as an important regulatory node by limiting the accumulation of PIP3 a necessary component for Akt activation. This regulation allows for controlled signal propagation that influences immune cell activity and development. SHIP-1 also interfaces with proteins like PTEN and SHP-1 which similarly modulate downstream signaling events within these pathways ensuring the maintenance of immune equilibrium.

SHIP-1 is associated with conditions like leukemia and autoimmune diseases. Alterations or deficiencies in SHIP-1 function can lead to abnormal immune responses or uncontrolled cell proliferation. For instance its reduced activity has connections to leukemia where there is excessive cell growth due to unchecked PI3K signaling. Moreover SHIP-1 is associated with autoimmune disorders through interactions with other signaling proteins that might affect the intensity and duration of immune responses highlighting its role in maintaining immune tolerance and preventing self-reactivity.
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Product protocols

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Target data

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed : 10764818, PubMed : 8723348, PubMed : 8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed : 10764818, PubMed : 8769125, PubMed : 9108392). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed : 16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.
See full target information INPP5D phospho S591
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websiteProtocolBooklet
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