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AB279926

Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit

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Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit is a ELISA for the measurement of Phospho-SHP2 (Y542) and Total SHP2 in Human, Mouse, Rat in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit (AB279926)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit (AB279926)

NIH/3T3 cells treated with/without PDGF.

NIH/3T3 cells were untreated or treated with 50 ng/ml PDGFBB for 10 mins.

Cell lysates were analyzed using this phospho ELISA and Western Blot.

Sandwich ELISA - Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit (AB279926)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit (AB279926)

Positive Control.

NIH/3T3 cells were treated with PDGFBB at 37°C for 10 mins.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human, Mouse, Rat

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-SHP2 (Y542) and Total SHP2 ELISA Kit (ab279926) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human, Mouse and Rat cell lysates. By determining phosphorylated SHP2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human, Mouse and Rat phospho-SHP2 and total SHP2. An anti-pan SHP2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SHP2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho SHP2 (Y542) antibody is added to detect phosphorylated SHP2. In the remaining wells, biotinylated anti-pan-SHP2 antibody is used to detect pan SHP2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SHP2 (Y542) or pan SHP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279926-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279926-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-SHP2 Coated Microplate", "number":"AB279926-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-SHP2 (Y542)-antibody", "number":"AB279926-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279926-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279926-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279926-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279926-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB279926-CMP02", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279926-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - NIH/3T3 cell lysate", "number":"AB279926-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-SHP2 detection antibody", "number":"AB279926-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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