Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 in Mouse in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The heterodimer with SLC3A2 functions as a sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, histidine, met hionine, tryptophan, valine, isoleucine and alanine (By similarity). The heterodimer with SLC3A2 mediates the uptake of L-DOPA and leucine (PubMed:11011012, PubMed:9915839). Functions as an amino acid exchanger (By similarity). May play a role in the transport of L-DOPA across the blood-brain barrier (PubMed:11011012). May act as the major transporter of tyrosine in fibroblasts (By similarity). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity). Can mediate the transport of thyroid hormones diiodothyronine (T2), triiodothyronine (T3) and thyroxine (T4) across the cell membrane. When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane (By similarity).
Additional Targets
Slc7a5
Alternative names
Lat1, Slc7a5, Large neutral amino acids transporter small subunit 1, 4F2 light chain, L-type amino acid transporter 1, Solute carrier family 7 member 5, 4F2 LC, 4F2LC, LAT1
What's included?
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Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 in Mouse in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Semi-quantitative
- Reactive species
- Mouse
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit (ab279846) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SLC7A5/LAT1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SLC7A5/LAT1 and total SLC7A5/LAT1. An anti-pan SLC7A5/LAT1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SLC7A5/LAT1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, mouse anti-phospho SLC7A5/LAT1 (Y161) antibody is added to detect phosphorylated SLC7A5/LAT1. In the remaining wells, mouse anti-pan-SLC7A5/LAT1 antibody is used to detect pan SLC7A5/LAT1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SLC7A5/LAT1 (Y161) or pan SLC7A5/LAT1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SLC7A5/LAT1 or LAT1 transporter also known as the large neutral amino acid transporter small subunit 1 plays an important role in facilitating the transport of large neutral amino acids across cell membranes. LAT1 is a heterodimer with a heavy chain known usually as SLC3A2 forming a functional transporter which is around 40-50 kDa in mass. LAT1 is highly expressed in tissues with high metabolic rates like the brain placenta and some cancer cells making it important for nutrient transport in these areas.
- Biological function summary
The LAT1 transporter mediates the uptake of essential amino acids which are critical for protein synthesis and cellular metabolism. It functions as part of the LAT1-SLC3A2 heterodimer complex ensuring effective transport across the blood-brain barrier and into other metabolically active tissues. This activity supports cellular growth and proliferation underlining its importance in rapidly dividing cells such as in cancer.
- Pathways
LAT1 plays a critical role in the mTOR signaling pathway which is central to regulating cell growth and metabolism in response to nutrient availability. LAT1's transport function influences the activation of mTORC1 by ensuring a steady supply of leucine an amino acid that activates this complex. LAT1 is also integral to the cross-pathway interaction with the insulin signaling pathway enhancing nutrient-dependent growth signals in conjunction with other transporters.
- Associated diseases and disorders
Abnormal LAT1 function links closely with cancer and neurological disorders. Its overexpression often marks various tumors where it supports cancer cell survival by sustaining amino acid supply often in conjunction with increased SLC3A2 expression. In neurological contexts LAT1 impairments can contribute to disorders involving neurotransmitter imbalances as it participates in amino acid transport critical for neurotransmitter synthesis.
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3 product images
Sandwich ELISA - Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit (ab279846)
Positive Control.
Jurkat cells were treated with Pervanadate.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Sandwich ELISA - Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit (ab279846)
Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with Pervanadate.
Sandwich ELISA - Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit (ab279846)
Jurkat cells treated with/without Pervanadate. Jurkat cells were untreated or treated with Pervanadate.
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