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AB279928

Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit

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Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit is a ELISA for the measurement of Phospho-Smad1 (S463/465) and Total Smad1 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit (AB279928)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit (AB279928)

Positive Control.

HeLa cells were treated with BMP2

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit (AB279928)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit (AB279928)

HeLa cells treated with/without BMP2.

HeLa cells were untreated or treated with BMP2. Cell lysates were analyzed using this phosphoELISA and Western Blot.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB226821

Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-20]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-20] (AB226821)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-Smad1 (S463/465) and Total Smad1 ELISA Kit (ab279928) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Smad1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Smad1 and total Smad1. An anti-pan Smad1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Smad1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Smad1 (S463/465) antibody is added to detect phosphorylated Smad1. In the remaining wells, rabbit anti-pan-Smad1 antibody is used to detect pan Smad1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Smad1 (S463/465) or pan Smad1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279928-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279928-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279928-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-Smad1 Coated Microplate", "number":"AB279928-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279928-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279928-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279928-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-pan-Smad1 detection antibody", "number":"AB279928-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-Smad1 (S463/465)-antibody", "number":"AB279928-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279928-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279928-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad1 also known as Mothers against decapentaplegic homolog 1 (MADH1) is a protein encoded by the SMAD1 gene in humans. It has a molecular mass of approximately 53 kDa and functions as a receptor-regulated Smad protein. Smad1 primarily transmits signals from the bone morphogenetic proteins (BMPs) a group of growth factors and cytokines. Expression of Smad1 occurs in various tissues including the lung heart and kidney. It plays an important role in mediating signaling pathways within different cellular environments.
Biological function summary

Smad1 participates in the transmission of BMP signals from the cell surface to the nucleus ensuring transcriptional regulation of target genes. In the presence of BMPs Smad1 forms complexes with Smad4 upon activation. These Smad complexes then translocate to the nucleus where they regulate gene expression. Smad1 influences cellular responses such as proliferation differentiation and apoptosis. In particular it plays a significant role in bone development and osteogenesis.

Pathways

Smad1 plays an important role within the BMP signaling pathway which is important for early development and tissue homeostasis. Within this pathway BMPs trigger the phosphorylation of Smad1 which then associates with Smad4 to propagate downstream signaling. Another associated pathway includes the TGF-beta signaling pathway where Smads like Smad2 and Smad3 show functional similarities and differences with Smad1. The interactions of Smad1 with related proteins like Smad4 highlight its significant contribution to cellular processes regulated by these pathways.

Smad1 associates with various pathological conditions. For instance aberrant Smad1 signaling has implications in cancer particularly in processes like tumor progression and metastasis. Disruptions in the BMP pathway involving Smad1 and Smad4 can influence the onset and development of disorders such as pulmonary hypertension where inappropriate cell growth and remodeling occur. Understanding Smad1's role in these contexts provides insights into potential therapeutic avenues for treatment and management of these conditions.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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