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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Phospho-Smad4 (T277) and Total Smad4 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-Smad4 (T277) and Total Smad4 in Human in Cell Lysate samples.
Colorimetric
Cell Lysate
Semi-quantitative
Human
Application | Reactivity | Dilution info | Notes |
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Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Phospho-Smad4 (T277) and Total Smad4 ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-Smad4 (T277) and Total Smad4 in Human in Cell Lysate samples.
Colorimetric
Cell Lysate
Semi-quantitative
Human
Pre-coated microplate (12 x 8 well strips)
Blue Ice
-20°C
-20°C
-20°C
Phospho-Smad4 (T277) and Total Smad4 ELISA Kit (ab279932) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Smad4 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Smad4 and total Smad4. An anti-pan Smad4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Smad4 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Smad4 (T277) antibody is added to detect phosphorylated Smad4. In the remaining wells, biotinylated anti-pan-Smad4 antibody is used to detect pan Smad4. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Smad4 (T277) or pan Smad4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Smad4 influences cell proliferation differentiation and apoptosis by mediating signals from TGF-beta cytokines. It is part of the Smad protein family acting as a transcriptional controller. Upon TGF-beta receptor activation Smad4 forms complexes with Smad2 and Smad3 translocating to the nucleus to regulate genes imperative for cellular homeostasis. Its role in cell cycle regulation underlines its contribution to normal cellular functions and its potential involvement in disorders.
Smad4 also known as DPC4 or MADH4 is a central protein in the TGF-beta signaling pathway with a molecular mass of approximately 60 kDa. It acts as a signal transducer that facilitates communication from the cell surface to the nucleus. Smad4 is broadly expressed in various tissues playing an important role in the regulation of cellular processes. It forms a complex with receptor-regulated Smads (R-Smads) to translocate to the nucleus where it influences gene transcription.
Smad4 operates within the TGF-beta pathway linking extracellular signals to nuclear transcription alterations. It participates in the regulation of epithelial-mesenchymal transition (EMT) a process important for development and tumor progression. In these pathways Smad4 interacts closely with Smad2 and Smad3 orchestrating various cellular responses to external stimuli through transcriptional management.
Smad4 is highly related to cancer and juvenile polyposis syndrome. Mutations or deletions in Smad4 disrupt its function contributing to the progression of pancreatic cancer and colorectal cancer among others. Within these contexts Smad4 connects strongly to other proteins like p21 and cyclin-dependent kinase inhibitors which are important in cell cycle arrest and impede tumor growth.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Positive Control.
Jurkat cells were treated with PV (Pervanadate).
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
Jurkat cells treated with/without CPT.
Jurkat cells were untreated or treated with CPT. Cell lysates were analyzed using this phosphoELISA and Western Blot.
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