Phospho-Src (Y419) and Total Src ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-Src (Y419) and Total Src ELISA Kit (AB279938)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were untreated or treated with pervanadate for 10 mins.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-Src (Y419) and Total Src ELISA Kit (AB279938)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 min.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
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Reactivity data
Product details
Phospho-Src (Y419) and Total Src ELISA Kit (ab279938) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated Src protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Src and total Src. An anti-pan Src antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Src present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Src (Y419) antibody is added to detect phosphorylated Src. In the remaining wells, rabbit anti-pan-Src antibody is used to detect pan Src. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Src (Y419) or pan Src bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
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Appropriate long-term storage conditions
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Src protein interacts with other proteins to modulate cell adhesion motility and angiogenesis forming part of larger protein complexes. Src phosphorylates specific tyrosine residues on its substrates altering their activity interaction or stability. This activity positions Src as an important factor in managing cell communication and structural organization. Src's interaction with focal adhesion complexes emphasizes its functionality in cellular structural integrity and intracellular communication pathways.
Pathways
Src plays a critical role in the integrin and growth factor receptor signaling pathways mediating cross-talk between cell surface receptors and intricate signaling cascades. It closely associates with focal adhesion kinase (FAK) within these pathways influencing cytoskeletal rearrangements and cell movement. Src's function in the epidermal growth factor receptor (EGFR) signaling pathway likewise demonstrates its importance in regulating cellular proliferation and survival mechanisms.
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