Phospho-Src (Y419) Quantitative ELISA Kit is a Quantitative ELISA kit for the measurement of Phospho-Src (Y419) Quantitative in Human in Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
What's included?
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Phospho-Src (Y419) Quantitative ELISA Kit is a Quantitative ELISA kit for the measurement of Phospho-Src (Y419) Quantitative in Human in Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Human
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Phospho-Src (Y419) Quantitative (ab279937) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Src protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human phospho-Src. An anti-pan Src antibody has been coated onto a 96-well plate. Standard and Samples are pipetted into the wells and Src present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-Src (Y419) antibody is used to detect phosphorylated Src. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Src (Y419) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Src also known as c-Src is a protein-tyrosine kinase involved in the regulation of many cellular processes. The molecular weight of Src is approximately 60 kDa. Src is ubiquitously expressed in human tissues but shows increased expression in specific tissues like the brain and epithelial cells. This protein has several important roles in cellular signal transduction particularly influencing cell growth differentiation and survival.
- Biological function summary
The Src protein interacts with other proteins to modulate cell adhesion motility and angiogenesis forming part of larger protein complexes. Src phosphorylates specific tyrosine residues on its substrates altering their activity interaction or stability. This activity positions Src as an important factor in managing cell communication and structural organization. Src's interaction with focal adhesion complexes emphasizes its functionality in cellular structural integrity and intracellular communication pathways.
- Pathways
Src plays a critical role in the integrin and growth factor receptor signaling pathways mediating cross-talk between cell surface receptors and intricate signaling cascades. It closely associates with focal adhesion kinase (FAK) within these pathways influencing cytoskeletal rearrangements and cell movement. Src's function in the epidermal growth factor receptor (EGFR) signaling pathway likewise demonstrates its importance in regulating cellular proliferation and survival mechanisms.
- Associated diseases and disorders
Src has significant implications in oncogenesis particularly in colorectal and breast cancers. Overexpression or abnormal activity of Src associates with tumor progression and metastasis. Within these cancers Src cooperates with various proteins like the EGFR amplifying aberrant cell signaling that contributes to uncontrolled cell growth. Investigating Src's role and regulation could offer insights into novel therapeutic strategies for controlling Src activity in cancer treatment.
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2 product images
Sandwich ELISA - Phospho-Src (Y419) Quantitative ELISA Kit (ab279937)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Sandwich ELISA - Phospho-Src (Y419) Quantitative ELISA Kit (ab279937)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were untreated or treated with Pervanadate for 10 mins.
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Product protocols
- Visit the general protocols
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