Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit
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- sELISA
Supplier Data
Sandwich ELISA - Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit (AB279948)
Positive Control.
A431 cells were treated with recombinant human EGF at 37°C for 20 min.
Cells were solubilzed at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit (AB279948)
A431 cells treated with/without EGF.
A431 cells were treated or untreated with EGF.
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Reactivity data
Product details
Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit (ab279948) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated STAT6 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-STAT6 and total STAT6. An anti-pan STAT6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT6 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho STAT6 (Y641) antibody is added to detect phosphorylated STAT6. In the remaining wells, mouse anti-pan-STAT6 antibody is used to detect pan STAT6. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT6 (Y641) or pan STAT6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.
Pathways
STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.
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