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AB279959

Phospho-TrkB (Y816) and Total TrkB ELISA Kit

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Phospho-TrkB (Y816) and Total TrkB ELISA Kit is a ELISA for the measurement of Phospho-TrkB (Y816) and Total TrkB in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (AB279959)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (AB279959)

U251 cells untreated/treated with Pervanadate.

U-251 cells were treated with Pervanadate.

Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (AB279959)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (AB279959)

Positive Control.

U-251 cells were treated with Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-TrkB (Y816) and Total TrkB ELISA Kit (ab279959) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated TrkB protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-TrkB and total TrkB. An anti-pan TrkB antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and TrkB present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho TrkB (Y816) antibody is added to detect phosphorylated TrkB. In the remaining wells, biotinylated anti-pan-TrkB antibody is used to detect pan TrkB. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TrkB (Y816) or pan TrkB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279959-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279959-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279959-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279959-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB279959-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279959-CMP07", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279959-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-TrkB detection antibody", "number":"AB279959-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - U-251 cell lysate", "number":"AB279959-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279959-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-TrkB (Y816)-antibody", "number":"AB279959-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-TrkB Coated Microplate", "number":"AB279959-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.
Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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