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Phospho-TrkB (Y816) and Total TrkB ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-TrkB (Y816) and Total TrkB in Human in Cell Lysate samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

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Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

What's included?

1 x 96 Tests
Components
100X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
2X Cell lysate buffer
1 x 5 mL
5X Assay Diluent
1 x 15 mL
Biotinylated anti-pan-TrkB detection antibody
1 x 1 Vial
HRP-conjugated anti-rabbit IgG concentrate (500X)
1 x 25 µL
Pan-TrkB Coated Microplate
1 x 1 Unit
Positive Control - U-251 cell lysate
1 x 1 Vial
Rabbit anti-phospho-TrkB (Y816)-antibody
1 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Phospho-TrkB (Y816) and Total TrkB ELISA Kit is a Semi-quantitative ELISA kit for the measurement of Phospho-TrkB (Y816) and Total TrkB in Human in Cell Lysate samples.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Assay type

Semi-quantitative

Reactive species

Human

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Phospho-TrkB (Y816) and Total TrkB ELISA Kit (ab279959) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated TrkB protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-TrkB and total TrkB. An anti-pan TrkB antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and TrkB present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho TrkB (Y816) antibody is added to detect phosphorylated TrkB. In the remaining wells, biotinylated anti-pan-TrkB antibody is used to detect pan TrkB. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TrkB (Y816) or pan TrkB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Activity summary

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

Associated diseases and disorders

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (ab279959), expandable thumbnail

    Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (ab279959)

    Positive Control.

    U-251 cells were treated with Pervanadate.

    Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

    Serial dilutions of lysates were analyzed in this ELISA.

  • Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (ab279959), expandable thumbnail

    Sandwich ELISA - Phospho-TrkB (Y816) and Total TrkB ELISA Kit (ab279959)

    U251 cells untreated/treated with Pervanadate.

    U-251 cells were treated with Pervanadate.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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