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AB279963

Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit

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Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit is a ELISA for the measurement of Phospho-TYK2 (Y1054) and Total TYK2 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit (AB279963)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit (AB279963)

Positive Control.

Jurkat cells were treated with Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit (AB279963)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit (AB279963)

Jurkat cells treated with/without Pervanadate.

Jurkat cells were treated with Pervanadate.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB303500

Anti-TYK2 antibody [EPR24628-106]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-TYK2 antibody [EPR24628-106] (AB303500)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phospho-TYK2 (Y1054) and Total TYK2 ELISA Kit (ab279963) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated TYK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-TYK2 and total TYK2. An anti-pan TYK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and TYK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho TYK2 (Y1054) antibody is added to detect phosphorylated TYK2. In the remaining wells, biotinylated anti-pan-TYK2 antibody is used to detect pan TYK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TYK2 (Y1054) or pan TYK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279963-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279963-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X Streptavidin-Conjugated HRP", "number":"AB279963-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rabbit anti-phospho-TYK2 (Y1054)-antibody", "number":"AB279963-CMP07", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279963-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279963-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279963-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279963-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-TYK2 Coated Microplate", "number":"AB279963-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279963-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279963-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Biotinylated anti-pan-TYK2 detection antibody", "number":"AB279963-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.
Biological function summary

TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.

Pathways

TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.

Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

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