JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279962

Phospho-TYK2 (Y1054) ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Phospho-TYK2 (Y1054) ELISA Kit is a ELISA for the measurement of Phospho-TYK2 (Y1054) in Human in Cell/Tissue Extracts samples.

View Alternative Names

Non-receptor tyrosine-protein kinase TYK2, TYK2

2 Images
Sandwich ELISA - Phospho-TYK2 (Y1054) ELISA Kit (AB279962)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-TYK2 (Y1054) ELISA Kit (AB279962)

Jurkat cells treated with/without Pervanadate.

Jurkat cells were treated with Pervanadate.

Sandwich ELISA - Phospho-TYK2 (Y1054) ELISA Kit (AB279962)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-TYK2 (Y1054) ELISA Kit (AB279962)

Jurkat cells were treated with Pervanadate.

Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB279939

Phospho-STAT2 (Y689) ELISA Kit

Sandwich ELISA - Phospho-STAT2 (Y689) ELISA Kit (AB279939)

  • 0
  • 0
Assay Kits

AB279948

Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit

Sandwich ELISA - Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit (AB279948)

  • 0
  • 0
Assay Kits

AB205715

STAT 5 A/B (pY694/699 + Total) ELISA Kit

SimpleStep

Sandwich ELISA - STAT 5 A/B (pY694/699 + Total) ELISA Kit (AB205715)

  • 0
  • 0
Primary Antibodies

AB303500

Anti-TYK2 antibody [EPR24628-106]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-TYK2 antibody [EPR24628-106] (AB303500)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phospho-TYK2 (Y1054) ELISA Kit (ab279962) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated TYK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-TYK2. An anti-pan TYK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and TYK2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-TYK2 (Y1054) antibody is used to detect phosphorylated TYK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TYK2 (Y1054) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279962-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279962-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - Jurkat cell lysate", "number":"AB279962-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279962-CMP09", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (1000X)", "number":"AB279962-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279962-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279962-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-TYK2 (Y1054)-antibody", "number":"AB279962-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-TYK2 Coated Microplate", "number":"AB279962-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279962-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.
Biological function summary

TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.

Pathways

TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.

Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.
style
left-column

Product protocols

style
left-column

Target data

Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity (PubMed : 10542297, PubMed : 10995743, PubMed : 7657660, PubMed : 7813427, PubMed : 8232552). Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling (PubMed : 10542297). Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed : 10542297, PubMed : 7638186). The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2 (PubMed : 10542297, PubMed : 25762719, PubMed : 7526154, PubMed : 7813427). In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members (PubMed : 7526154, PubMed : 7657660). In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes (PubMed : 10542297, PubMed : 25762719, PubMed : 7657660). Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed : 29162862).
See full target information TYK2 phospho Y1054
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com