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AB279969

Phospho-WNK1 (T60) and Total WNK1 ELISA Kit

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Phospho-WNK1 (T60) and Total WNK1 ELISA Kit is a ELISA for the measurement of Phospho-WNK1 (T60) and Total WNK1 in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-WNK1 (T60) and Total WNK1 ELISA Kit (AB279969)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-WNK1 (T60) and Total WNK1 ELISA Kit (AB279969)

Positive Control.

T47D cells were treated with IGF-1 at 37°C for 30 min.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-WNK1 (T60) and Total WNK1 ELISA Kit (AB279969)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-WNK1 (T60) and Total WNK1 ELISA Kit (AB279969)

HeLa cells treated with/without IGF-1.

HeLa cells were untreated or treated with IGF-1 (50 ng/ml) for 30 min.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Results type

Semi-Quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB174854

Anti-WNK1 antibody [EPR2743(3)]

RabMAb|Recombinant|KO Validated

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Primary Antibodies

AB303518

Anti-NKCC1 antibody [EPR26256-60]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] (AB303518)

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Proteins & Peptides

AB125579

Recombinant Human OXSR1 protein

SDS-PAGE - Recombinant Human OXSR1 protein (AB125579)

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Proteins & Peptides

AB177262

Recombinant human WNK1 protein (Tagged)

Functional Studies - Recombinant human WNK1 protein (Tagged) (AB177262)

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Reactivity data

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Product details

Phospho-WNK1 (T60) and Total WNK1 ELISA Kit (ab279969) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated WNK1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-WNK1 and total WNK1. An anti-pan WNK1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and WNK1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho WNK1 (T60) antibody is added to detect phosphorylated WNK1. In the remaining wells, rabbit anti-pan-WNK1 antibody is used to detect pan WNK1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WNK1 (T60) or pan WNK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WNK1 also known as With No Lysine 1 is a protein kinase with a molecular mass of approximately 251 kDa. Mechanically WNK1 functions as a serine/threonine kinase influencing the phosphorylation status of various substrates involved in ionic balance and cell volume regulation. It plays an important role in controlling ion transport channels. The protein is expressed in multiple tissues with high levels in the kidney and lower levels in the brain heart and other regions of the body. Its activity is especially important in the distal nephrons of the kidney.
Biological function summary

WNK1 affects sodium and potassium ion transport which is essential for maintaining homeostasis. It is a part of a larger signaling complex where it modulates the activity of SPAK and OSR1 kinases which directly affect ion transporters like NKCCs and NCC. By regulating these kinases WNK1 contributes to maintaining the balance of electrolytes influencing blood pressure and general cellular osmoregulation.

Pathways

WNK1 significantly impacts the WNK-SPAK/OSR1 signaling pathway which has direct roles in sodium and potassium ion transport. The pathway associates closely with proteins like SPAK and OSR1 which WNK1 activates affecting the function of ion transporters and channels. This signaling mechanism plays an integral role in renal salt reabsorption and is important for controlling systemic blood pressure.

Mutations and dysregulation of WNK1 link to hereditary hypertension and pseudohypoaldosteronism type II (PHAII) also known as Gordon's syndrome. These conditions involve elevated blood pressure and electrolyte imbalance due to altered transport activity. A change in WNK1 function can disrupt normal interaction with other proteins in its pathway such as NCC leading to these disorders. Understanding WNK1's role offers potential therapeutic targets for managing conditions related to electrolyte imbalance and blood pressure regulation.
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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