JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279972

Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-ZAP70 (Y493) and Total ZAP70 in Human in Cell/Tissue Extracts samples.
2 Images
Sandwich ELISA - Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit (AB279972)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit (AB279972)

Positive Control.

Jurkat cells were treated with Pervanadate.

Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit (AB279972)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit (AB279972)

Jurkat cells treated with/without Pervanadate.

Jurkat cells were untreated or treated with PV.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Semi-quantitative

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit (ab279972) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ZAP70 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human phospho-ZAP70 and total ZAP70. An anti-pan ZAP70 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ZAP70 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, Mouse anti-phospho-ZAP70 (Tyr493) antibody is added to detect phosphorylated ZAP70. In the remaining wells, Mouse anti-pan-ZAP70 antibody is used to detect pan ZAP70. After washing away unbound antibody, HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ZAP70 (Tyr493) or pan ZAP70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279972-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 µL", "name":"HRP-conjugated anti-mouse IgG concentrate (1000X)", "number":"AB279972-CMP14", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279972-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279972-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-ZAP70 Coated Microplate", "number":"AB279972-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse anti-pan-ZAP70 detection antibody", "number":"AB279972-CMP13", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse anti-phospho-ZAP70 (Tyr493) antibody", "number":"AB279972-CMP12", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279972-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279972-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - treated Jurkat cell lysate", "number":"AB279972-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279972-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZAP70 also known as Zeta-chain-associated protein kinase 70 is a tyrosine kinase that plays an important role in T-cell receptor signaling. The protein has a molecular mass of approximately 70 kDa. ZAP70 is mainly expressed in T cells and natural killer (NK) cells. It gets activated upon T-cell receptor engagement initiating downstream signaling cascades essential for T-cell function and adaptive immune response.
Biological function summary

ZAP70 is instrumental in the signaling cascade that activates T cells in response to antigen recognition. It forms part of a larger signaling complex following the engagement of the T-cell receptor with antigens. This complex transmits critical activation signals leading to further cellular responses such as cytokine production and cell proliferation. ZAP70's function ensures appropriate immune responses and helps maintain immune system balance.

Pathways

ZAP70 plays an integral role in T-cell receptor signaling pathways. This protein interacts with other signaling molecules like Lck and LAT facilitating the transmission of activation signals within the cell. As part of the T-cell activation pathway ZAP70 helps to bridge receptor-ligand interactions with cellular responses enabling the immune cells to combat infections effectively.

ZAP70 mutations or dysregulations can lead to severe immunodeficiencies or autoimmune diseases. For example ZAP70 deficiency results in severe combined immunodeficiency (SCID) which severely impairs immune function. Additionally aberrant ZAP70 activity has links to certain autoimmune disorders like rheumatoid arthritis where it influences abnormal immune signaling. Understanding the biochemical pathways that involve ZAP70 provides insight into managing these diseases and developing therapeutic strategies.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB260069

Human PNP ELISA Kit (Purine Nucleoside Phosphorylase)

Recombinant|SimpleStep

Sandwich ELISA - Human PNP ELISA Kit (Purine Nucleoside Phosphorylase) (AB260069)

  • 0
  • 0
Primary Antibodies

AB247258

Anti-ZAP70 antibody [YE291] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 antibody [YE291] - BSA and Azide free (AB247258)

  • 0
  • 0
Proteins & Peptides

AB204139

Recombinant human ZAP70 (mutated Y319F) protein

Functional Studies - Recombinant human ZAP70 (mutated Y319F) protein (AB204139)

  • 0
  • 0
Cell Lines & Lysates

AB273841

Human ZAP70 knockout Jurkat cell line

Advanced Validation

Western blot - Human ZAP70 knockout Jurkat cell line (AB273841)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com