Phosphotyrosine BTK ELISA Kit
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Supplier Data
Sandwich ELISA - Phosphotyrosine BTK ELISA Kit (AB279750)
Positive Control.
THP1 cells were treated with Pervanadate at 37°C for 10 min.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- ELISA
Supplier Data
ELISA - Phosphotyrosine BTK ELISA Kit (AB279750)
U937 cells untreated/treated with Pervanadate.
U937 cells were untreated or treated with Pervanadate for 10 mins at 37°C.
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Reactivity data
Product details
Phosphotyrosine BTK ELISA Kit (ab279750) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated BTK in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This sandwich-based ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-BTK. An anti-BTK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated BTK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-BTK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
BTK plays an important role in the immune response by transmitting signals from the B cell receptor to the inside of the cell which promotes B cell maturation and survival. It operates as part of a larger signal transduction complex that includes other proteins and molecules. For accurate quantification of BTK expression a BTK sandwich ELISA kit may be utilized capturing and revealing the BTK protein's presence in sample preparations.
Pathways
BTK associates with both the B cell receptor signaling and the PI3K-Akt pathway. These pathways are critical for the proper functioning and proliferation of B cells. BTK interacts with proteins like PLCγ2 and BLNK in the signaling cascade highlighting its central role in transmitting extracellular signals to elicit appropriate cellular responses.
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